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MABN2471-100UG Anti-Aquaporin-4 Antibody, clone mECD

Anti-Aquaporin-4, clone mECD, Cat. No. MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation.

Anti-Aquaporin-4 Antibody, clone mECD MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: AQP4 UniProt Number: P55087
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MABN2471-100UG
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MFC, IHC, IF, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2471-100UG
      DescriptionAnti-Aquaporin-4 Antibody, clone mECD
      Alternate Names
      • AQP-4
      • Mercurial-insensitive water channel
      • MIWC
      • WCH4
      Background InformationAquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NMO) from multiple sclerosis. Clone mECD is shown to induce clustering and internalization of Aquporin-4 and cause its redistribution, which is also observed with NMO-IgG. Exposure to mECD antibody is also shown to cause coaggregation of astrocytic CD28B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl.Acad. Sci. USA 114(21); 5491-5496).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Applications
      ApplicationAnti-Aquaporin-4, clone mECD, Cat. No. MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesFlow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

      Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
      Biological Information
      ImmunogenHis-tagged full-length human recombinant Aquaporin-4.
      Epitopeextracellular domain
      ClonemECD
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AQP4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight34.83 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat brain frozen tissue sections.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in Rat brain frozen tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2471-100UG 04054839551222