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MABE1138-25UL Anti-BAF180 Antibody, clone 370.1.8C3

Anti-BAF180, clone 370.1.8C3, Cat. No. MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting.

Anti-BAF180 Antibody, clone 370.1.8C3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: PBRM1, BAF180, PB1 UniProt Number: Q86U86
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MABE1138-25UL
25 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1138-25UL
      DescriptionAnti-BAF180 Antibody, clone 370.1.8C3
      Alternate Names
      • Protein polybromo-1
      • hPB1
      • BRG1-associated factor 180
      • Polybromo-1D
      Background InformationProtein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a large, chromatin-remodeling protein that contains six bromodomains, two BAH-domains, and one HMG-box. Bromodomains recognize and bind to acetylated lysines, the BAH (bromo-adjacent homology) domains mediate protein protein interactions, and the HMG-box domain binds to DNA. Nine isoforms of BAF180 have been described that are produced by alternative splicing. BAF180 serves as a subunit of the SWI/SNF complex and plays critical roles in the regulation of chromatin remodeling and gene transcription, and is reported to be frequently mutated in several human cancers. BAF180 acts asa negative regulator of cell proliferation and plays a role in suppression of tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter. Mutations in PBRM1 gene are known to cause renal cell carcinoma. (Ref.: Lee, H., et al. (2016). Oncotarget 7(15);19134-46; Gao, W., et al. (2017). Proc. Natl. Acad. Sci. USA. 114(5):1027-1032).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BAF180, clone 370.1.8C3, Cat. No. MABE1138, is a highly specific rat monoclonal antibody that detects Protein Polybromo-1 (BAF180) and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected BAF180 in Western Blotting applications (Gao, W., et. al. (2017). Proc Natl Acad Sci U S A. 114(5):1027-1032).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 241 amino acids from the C-terminal half of human Protein polybromo-1 (BAF180).
      Clone370.1.8C3
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 370.1.8C3 is a rat monoclonal antibody that specifically detects human Protein Polybromo-1 (BAF180). It targets an epitope within 241 amino acids from the C-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PBRM1
      • BAF180
      • PB1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~210 kDa observed; 192.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BAF180 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1138-25UL 04054839540271