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OP191 Anti-BCRP Mouse mAb (BXP-21)

Anti-BCRP Mouse mAb (BXP-21) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP191
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~70 kDa BCRP protein in VP cells.
      Catalogue NumberOP191
      Brand Family Calbiochem®
      Application Data
      Detection of human BCRP by immunocytochemistry. Samples: Parental IGROV1 ovarian tumor cell line (figure A) and the drug-selected BCRP-overexpressing subline T8 (figure B). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:50). Detection: fluorescence.

      Detection of human BCRP by immunohistochemistry. Samples: Placenta (figure A), liver (figure B), small intestine (figure C), colon (figure D), prostate (figure E), ovary (figure F), submucosa of the stomach (figure G), and choroid plexus (figure H) with anti-BCRP clone BXP-21 (figures D and H) or BXP-34 (figures A-C, E-G). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:20). Detection: chemiluminescence.
      References
      ReferencesScheffer, G.L., et al. 2000. Cancer Res. 60, 2589.
      Doyle, L.A., et al. 1998. Proc. Natl. Acad, Sci. USA 95, 15665.
      Product Information
      FormLiquid
      FormulationCulture supernatant, 0.7% BSA.
      Positive controlMCF7/AdrVP cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesFrozen Sections (1:20)
      Immunoblotting (1:50)
      Immunocytochemistry (1:20-1:50)
      Paraffin Sections (1:20, heat pre-treatment required)
      Application CommentsDoes not cross-react with human MDR1, MRP1, or MRP2. Formaldehyde-fixed, paraffin-embedded tissues require pre-treatment with 10 mM citric acid, pH 6.0, at 95°C for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 271-396 of human breast cancer resistance protein (BCRP) fused to maltose-binding protein
      ImmunogenHuman
      CloneBXP-21
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP191 0

      Documentation

      Anti-BCRP Mouse mAb (BXP-21) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-BCRP Mouse mAb (BXP-21) Certificates of Analysis

      TitleLot Number
      OP191

      References

      Reference overview
      Scheffer, G.L., et al. 2000. Cancer Res. 60, 2589.
      Doyle, L.A., et al. 1998. Proc. Natl. Acad, Sci. USA 95, 15665.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFrozen Sections (1:20)
      Immunoblotting (1:50)
      Immunocytochemistry (1:20-1:50)
      Paraffin Sections (1:20, heat pre-treatment required)
      Application Data
      Detection of human BCRP by immunocytochemistry. Samples: Parental IGROV1 ovarian tumor cell line (figure A) and the drug-selected BCRP-overexpressing subline T8 (figure B). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:50). Detection: fluorescence.

      Detection of human BCRP by immunohistochemistry. Samples: Placenta (figure A), liver (figure B), small intestine (figure C), colon (figure D), prostate (figure E), ovary (figure F), submucosa of the stomach (figure G), and choroid plexus (figure H) with anti-BCRP clone BXP-21 (figures D and H) or BXP-34 (figures A-C, E-G). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:20). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody supplied as culture supernatant. Recognizes the ~70 kDa BCRP.
      BackgroundBreast cancer resistance protein (BCRP) is a transmembrane half-transporter that plays a major role in the multidrug resistance phenotype of human breast cancer. Overexpression of BCRP actively reduces the intracellular drug concentration of some chemotherapeutics and their efficacy.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 271-396 of human breast cancer resistance protein (BCRP) fused to maltose-binding protein
      CloneBXP-21
      IsotypeIgG2a
      Specieshuman
      Positive controlMCF7/AdrVP cells
      FormLiquid
      FormulationCulture supernatant, 0.7% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with human MDR1, MRP1, or MRP2. Formaldehyde-fixed, paraffin-embedded tissues require pre-treatment with 10 mM citric acid, pH 6.0, at 95°C for 20 min. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesScheffer, G.L., et al. 2000. Cancer Res. 60, 2589.
      Doyle, L.A., et al. 1998. Proc. Natl. Acad, Sci. USA 95, 15665.