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PC89 Anti-Bcl-xs (Ab-1) (119-131) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC89
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~16 kDa Bcl-xs protein in doxorubicin-treated MCF-7 cells. Does not cross-react with Bcl-xL.
      Catalogue NumberPC89
      Brand Family Calbiochem®
      Application Data
      Detection of Bcl-xs by immunobloitting. Sample: Whole cell lysate from doxorubicin-treated MCF7 cells. Primary antibody: Anti-Bcl-xs (Ab-1) (119-131) Rabbit pAb (Cat. No. PC89) (2.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBargou, R.C., et al. 1995. Eur. J. Immunol. 25, 770.
      Dole, M.G., et al. 1995. Cancer Res. 55, 2576.
      Zhang,H., et al. 1995. Biochem. Biophys. Res. Commun. 208, 950.
      Fang, W., et al. 1994. J. Immunol. 153, 4388.
      Krajewski, S., et al. 1994. Cancer Res. 54, 5501.
      Nunex, G. et al. 1994. Immunol. Today 15, 582.
      Boise L.H., et al. 1993. Cell 74, 597.
      Cazais-Hatem, D.L., et al. 1992. Biochim. Biophys. Acta 1132, 109.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlDoxorubicin-induced MCF7 or HeLa cells or thymocytes
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml)
      Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application CommentsThis antibody will not react with bcl-xL, but additional higher molecular weight bands may be seen with some cell lines. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (AYQSFEQDTFVEL) corresponding to amino acids 119-131 of human Bcl-xS
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PC89 0

      Documentation

      Anti-Bcl-xs (Ab-1) (119-131) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Bcl-xs (Ab-1) (119-131) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC89

      References

      Reference overview
      Bargou, R.C., et al. 1995. Eur. J. Immunol. 25, 770.
      Dole, M.G., et al. 1995. Cancer Res. 55, 2576.
      Zhang,H., et al. 1995. Biochem. Biophys. Res. Commun. 208, 950.
      Fang, W., et al. 1994. J. Immunol. 153, 4388.
      Krajewski, S., et al. 1994. Cancer Res. 54, 5501.
      Nunex, G. et al. 1994. Immunol. Today 15, 582.
      Boise L.H., et al. 1993. Cell 74, 597.
      Cazais-Hatem, D.L., et al. 1992. Biochim. Biophys. Acta 1132, 109.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      ApplicationImmunoblotting (2.5 µg/ml)
      Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application Data
      Detection of Bcl-xs by immunobloitting. Sample: Whole cell lysate from doxorubicin-treated MCF7 cells. Primary antibody: Anti-Bcl-xs (Ab-1) (119-131) Rabbit pAb (Cat. No. PC89) (2.5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~16 kDa Bcl-xs protein.
      BackgroundBcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs2. The larger mRNA gives rise to a protein product, bcl-xL, which is similar in size and predicted structure to bcl-2. Like the long form, the short form (bcl-xs), contains a putative transmembrane domain which is responsible for its mitochondrial localization. The bcl-xs protein is generated following a deletion of 63 amino acids from the long form by the usage of a different 5' splice site in the first coding exon. The segment deleted corresponds to the region of highest homology among bcl-2 proteins derived from multiple species5. bcl-xs lacks the conserved BH1 and BH2 domains which are present in most members of the bcl-2 gene family. Studies have shown that bcl-xs can form heterodimers with bcl-2 and does so via codons 24-78. Tissue expression of bcl-xs is different from that seen for the long form of the protein, as is the regulation of the gene expression. bcl-xs appears to be critical in the development of the vertebrate immune system playing a central role in the establishment of the T cell compartment.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (AYQSFEQDTFVEL) corresponding to amino acids 119-131 of human Bcl-xS
      IsotypeIgG
      Specieshuman, not mouse, not rat
      Positive controlDoxorubicin-induced MCF7 or HeLa cells or thymocytes
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody will not react with bcl-xL, but additional higher molecular weight bands may be seen with some cell lines. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBargou, R.C., et al. 1995. Eur. J. Immunol. 25, 770.
      Dole, M.G., et al. 1995. Cancer Res. 55, 2576.
      Zhang,H., et al. 1995. Biochem. Biophys. Res. Commun. 208, 950.
      Fang, W., et al. 1994. J. Immunol. 153, 4388.
      Krajewski, S., et al. 1994. Cancer Res. 54, 5501.
      Nunex, G. et al. 1994. Immunol. Today 15, 582.
      Boise L.H., et al. 1993. Cell 74, 597.
      Cazais-Hatem, D.L., et al. 1992. Biochim. Biophys. Acta 1132, 109.