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MAB1759F Anti-CD59 Antibody, clone 2/24, FITC conjugated

Anti-CD59 Antibody, clone 2/24, FITC conjugated detects level of CD59 & has been published & validated for use in FC & IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD59, MACIF, MIN2, MIN3, PROTECTIN, HRF-20, 16.3A5, MSK21, EJ16, HRF20, EL32, G344, MIC11, MIN1, Protectin, MAC-IP, MGC2354, EJ30, MIRL, p18-20 UniProt Number: P13987
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MAB1759F
100 assays  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IFMFITCMonoclonal Antibody
      Description
      Catalogue NumberMAB1759F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD59 Antibody, clone 2/24, FITC conjugated
      OverviewBIOCHEMISTRY:

      p I = 6.1
      Alternate Names
      • Protectin
      References
      Product Information
      FormatFITC
      PresentationPurified immunoglobulin. FITC Conjugated. The antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CD59 Antibody, clone 2/24, FITC conjugated detects level of CD59 & has been published & validated for use in FC & IF.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      Application NotesThis monoclonal is useful in studies of complement and homologous restriction of complement action of the properties of GPI linked proteins and in the characterisation of cells from patients with PNH.

      SUGGESTED USAGE:
      Flow cytometry and immunofluorescence – use 10µL direct from the vial per 1x106 cells in a volume of 100 µL.
      For analysis of erythrocytes, whole blood samples should be diluted approximately 500 fold.
      For nucleated cells, use of the buffer coat will remove the burden of erythrocytes.
      Platelets may be harvested by low speed centrifugation.
      Biological Information
      ImmunogenHuman erythrocytes
      Clone2/24
      HostMouse
      SpecificityThis antibody binds to a 17-25kD glycoprotein (Fletcher et al., 1990a,b) which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane(Hadam, 1989). It is identical to other CD59 monoclonals, MEM-43 and YTH 53.1, defined at the 4th International Conference on Human Leucocyte Differentiation Antigens(Davies et al., 1989; Stefanova et al., 1989). CD59 is an inhibitor of attack by autologous complement(Ojcius et al., 1990) possibly by limiting incorporation of C9 into the membrane complex(Rollins & Sims, 1990; Meri et al., 1990). GPI linked proteins such as CD59 are deficient on cells from patients with the acquired clonal disorder paroxysmal nocturnal haemoglobulinuria (PNH) in which the erythrocytes are susceptible to lysis by complement and leading to anemia(Rosse, 1990). Evidence suggests that CD59 may be the most important complement regulatory protein missing in PNH(Fletcher et al., 1990; Yamashina et al., 1990).

      Cell reactivity (Fletcher et al., 1990a,b; Hadam, 1989):

      The antibody reacts with all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD59
      • MACIF
      • MIN2
      • MIN3
      • PROTECTIN
      • HRF-20
      • 16.3A5
      • MSK21
      • EJ16
      • HRF20
      • EL32
      • G344
      • MIC11
      • MIN1
      • Protectin
      • MAC-IP
      • MGC2354
      • EJ30
      • MIRL
      • p18-20
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13987 # Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
      SIZE: 128 amino acids; 14177 Da
      SUBUNIT: Interacts with T-cell surface antigen CD2.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
      PTM: N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. & Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
      SIMILARITY: SwissProt: P13987 ## Contains 1 UPAR/Ly6 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsSTORAGE

      Store at 2 to 8oC, for up to 6 months. For prolonged periods, store below -20oC in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

      WARNING

      The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1759F 04053252723636

      Documentation

      Anti-CD59 Antibody, clone 2/24, FITC conjugated SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD59 Antibody, clone 2/24, FITC conjugated Certificates of Analysis

      TitleLot Number
      Mouse Monoclonal antibody to Human CD59, FITC conjugated (ready to use) - 2029630 2029630
      Mouse Monoclonal antibody to Human CD59, FITC conjugated (ready to use) - ME9AN9517 ME9AN9517
      Mouse Monoclonal antibody to Human CD59, FITC conjugated (ready to use) - ME9BN0932 ME9BN0932
      Mouse monoclonal antibody to Human CD59 - 3674848 3674848
      Mouse monoclonal antibody to Human CD59 - 3730000 3730000
      Mouse monoclonal antibody to Human CD59, -2673105 2673105
      Mouse monoclonal antibody to Human CD59, FITC Conjugated (ready to use) 3118147
      Mouse monoclonal antibody to Human CD59, FITC Conjugated (ready to use) - 3378727 3378727

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      Alternative Format

      Catalog Number Description  
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies