Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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217620
Sigma-AldrichAnti-CD64 Mouse mAb (10.1)
Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
More>>Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies. Less<<
Anti-CD64 Mouse mAb (10.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoblotting (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments)
Application Comments
The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
human rheumatoid synovial fluid cells/monocytes
Immunogen
Human
Clone
10.1
Host
Mouse
Purity
≥95% by SDS-PAGE
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Anti-CD64 Mouse mAb (10.1) Certificates of Analysis
Title
Lot Number
217620
References
Reference overview
Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
25-July-2007 RFH
Application
Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoblotting (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments)
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
≥95% by SDS-PAGE
Preservative
0.5 mg/ml gentamycin sulfate
Comments
The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity
Standard Handling
References
Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.