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ST1548 Anti-CTH Mouse mAb (S51)

Anti-CTH Mouse mAb (S51) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cystathionase, Anti-Cystathionase γ-Lyase

ST1548
  
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      Overview

      Replacement Information

      Key Specifications Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~38 kDa CTH protein in K-562 cells and human pancreas tissue.
      Catalogue NumberST1548
      Brand Family Calbiochem®
      SynonymsAnti-Cystathionase, Anti-Cystathionase γ-Lyase
      Application Data
      Detection of human CTH by immunoblotting. Sample: Whole cell lysate from K-562 cells. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CTH by staining praffin sections. Sample: Formalin-fixed paraffin embedded human pancreas tissue. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (3 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesZhu, X.Y., et al. 2010. Cell Mol Life Sci. 67, 1119.
      Siebert, N., et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G1266.
      Product Information
      FormLiquid
      Formulationin PBS, pH 7.2
      Negative control293T cells
      Positive controlK-562 cells, Human pancreas tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human CTH (aa 1-406) expressed as a GST fusion protein
      CloneS51
      HostMouse
      IsotypeIgG1
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ST1548 0

      Documentation

      References

      Reference overview
      Zhu, X.Y., et al. 2010. Cell Mol Life Sci. 67, 1119.
      Siebert, N., et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G1266.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-Cystathionase, Anti-Cystathionase γ-Lyase
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human CTH by immunoblotting. Sample: Whole cell lysate from K-562 cells. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CTH by staining praffin sections. Sample: Formalin-fixed paraffin embedded human pancreas tissue. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~38 kDa CTH protein.
      BackgroundCTH is a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria.
      HostMouse
      ImmunogenFull-length, recombinant human CTH (aa 1-406) expressed as a GST fusion protein
      CloneS51
      IsotypeIgG1
      Specieshuman
      Positive controlK-562 cells, Human pancreas tissue
      Negative control293T cells
      FormLiquid
      Formulationin PBS, pH 7.2
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesZhu, X.Y., et al. 2010. Cell Mol Life Sci. 67, 1119.
      Siebert, N., et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G1266.