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07-1466 Anti-Cdc42 Antibody

Anti-Cdc42, Cat. No. 07-1466, is a rabbit polyclonal antibody that detects Cdc42 and is tested for use in Western Blotting.

Anti-Cdc42 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: CDC42, CDC42Hs, G25K UniProt Number: P60953
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07-1466
100 µg  
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      Overview

      Replacement Information
      Replacement Information07-1466 is a recommended replacement for AB4201

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1466
      ReplacesAB4201
      DescriptionAnti-Cdc42 Antibody
      Alternate Names
      • G25K
      • GTP-binding Protein
      Background InformationCell division control protein 42 homolog (UniProt: P60953; also known as EC:3.6.5.2, G25K GTP-binding protein, CDC42) is encoded by the CDC42 gene (Gene ID: 998) in human. Cdc42 is a plasma membrane-associated small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state. In its active state it binds to a variety of effector proteins to regulate cellular responses. Its activity is regulated by guanine nucleotide exchange factors (GEFs), which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) that increase the GTP hydrolysis activity, and GDP dissociation inhibitors, which inhibit the dissociation of the nucleotide from the GTPase. Cdc42 is known to regulate the dynamic organization of the cytoskeleton and membrane trafficking for physiologic processes such as cell proliferation, motility, polarity, cytokinesis, and growth. It is involved in epithelial cell polarization processes and regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. During the prometaphase stage, it localizes to spindle and moves to the central spindle as cells progress through anaphase to telophase. Cdc42 is also reported to play a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups. Deregulation of Cdc42 is found in several pathogenic processes such as cancer, cardiovascular diseases, and neuronal degenerative diseases. (Ref.: Schlam, D., et al. (2015). Nat. Commun. 6; 8623; Oceguera-Yanez, F., et al. (2005). J. Cell Biol. 168(2); 221-232).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat Brain Lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cdc42, Cat. No. 07-1466, is a rabbit polyclonal antibody that detects Cdc42 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Cdc42 in rat brain microsomal preparations.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal half of human Cdc42.
      EpitopeC-terminal half
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Cdc42. It targets an epitope within 12 amino acids from the C-terminal half. Please check our ZooMAb® version of Anti-Cdc42 (Cat. No. ZRB1848; Anti-Cdc42, clone 2D16 ZooMAb ® Rabbit Monoclonal).
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Zebra Fish, Canine, Porcine, Monkey, Feline, Bovine, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
      Gene Symbol
      • CDC42
      • CDC42Hs
      • G25K
      Purification MethodAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
      ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
      SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
      Molecular WeightTarget molecular weight: ~19 kDa observed; 21.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdc42 in Rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-1466 04053252737619

      Documentation

      Anti-Cdc42 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cdc42 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Cdc42 2475662
      Anti-Cdc42 - 2398896 2398896
      Anti-Cdc42 - 1973235 1973235
      Anti-Cdc42 - 2196115 2196115
      Anti-Cdc42 - 2299610 2299610
      Anti-Cdc42 - 2942451 2942451
      Anti-Cdc42 - 3387637 3387637
      Anti-Cdc42 - 3545027 3545027
      Anti-Cdc42 - JBC1794850 JBC1794850
      Anti-Cdc42 - NG1609556 NG1609556
      Show more

      References

      Reference overviewPub Med ID
      p16 Stimulates CDC42-dependent migration of hepatocellular carcinoma cells.
      Chen, YW; Chu, HC; Ze-Shiang Lin, ; Shiah, WJ; Chou, CP; Klimstra, DS; Lewis, BC
      PloS one  8  e69389  2013

      Show Abstract       		23894465 23894465