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MABE873 Anti-Chd2, clone 8H3 Antibody

This Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.

Anti-Chd2, clone 8H3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: Chd2 UniProt Number: E9PZM4
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MABE873
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICC, ChIPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE873
      DescriptionAnti-Chd2, clone 8H3 Antibody
      Alternate Names
      • SNF2 chromatin remodelling enzyme
      • Chromodomain Helicase DNA Binding Protein 2
      • Protein Chd2
      Background InformationChd2, also known as Chromodomain-helicase-DNA-binding protein 2 (CHD-2), or ATP-dependent helicase CHD2, and encoded by the gene CHD2, belongs to the CHD family of proteins. This family is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes are chromatin remodeling proteins and alter gene expression by modifying the chromatin structure of a particular gene. The alteration affects the access of the transcriptional apparatus to it, and thus the gene’s expression. In the mouse Chd2 is expressed in heart, forebrain, extremities, and facial and dorsal regions during specific times of embryonic development. Chd2 null mice are embryonic lethal, and in humans, various mutations in CHD-2 gene appear to cause a variety of disorders and developmental defects. Other mutations associated with the CHD2 gene locus appear to cause childhood-onset epileptic encephalopathy (EEOC).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Chd2 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

      Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse Chd2.
      Clone8H3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Chd2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~211 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. Two known isoforms may be observed at 50 kDa and 200 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Chd2 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE873 04053252955228

      Documentation

      Anti-Chd2, clone 8H3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Chd2, clone 8H3 - Q2383914 Q2383914
      Anti-Chd2, clone 8H3 - 3218874 3218874
      Anti-Chd2, clone 8H3 - 3407335 3407335
      Anti-Chd2, clone 8H3 - 3738081 3738081
      Anti-Chd2, clone 8H3 - 3810505 3810505
      Anti-Chd2, clone 8H3 - 3978231 3978231
      Anti-Chd2, clone 8H3 - 4077670 4077670
      Anti-Chd2, clone 8H3 - 4125701 4125701
      Anti-Chd2, clone 8H3 - 4169254 4169254
      Anti-Chd2, clone 8H3 -2659636 2659636
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      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics