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CC12F Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) Fluorescein Conjugate

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CC12F
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
      Catalogue NumberCC12F
      Brand Family Calbiochem®
      References
      ReferencesInaba, T., et al. 1992. Genomics 13, 565.
      Jiang, W., et al. 1992. Cancer Res. 2, 2980.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Xiong, Y., et al. 1992. Genomics 13, 575.
      Xiong, Y., et al. 1992. Cell 71, 504.
      Motokura, T., et al. 1991. Nature 350, 512.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Product Information
      FormLiquid
      FormulationIn 50% glycerol, 50% sodium phosphate buffer.
      Positive controlA431 or MCF7 cells
      PreservativeNone
      Applications
      Application ReferencesImmunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949. Bartkova, J., et al. 1994. J. Pathol. 172, 237. Lukas, J., et al. 1994. Oncogene 9, 707.
      Key Applications Flow Cytometry
      Immunofluorescence
      Application NotesFlow Cytometry (5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Application CommentsDoes not react with cyclins D2 or D3. For immunoblotting, immunohistochemistry, immunoprecipitation and neutralization studies, we recommend Cat. No. CC12. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human cyclin D1
      ImmunogenHuman
      CloneDCS-6
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CC12F 0

      Documentation

      Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) Fluorescein Conjugate Certificates of Analysis

      TitleLot Number
      CC12F

      References

      Reference overview
      Inaba, T., et al. 1992. Genomics 13, 565.
      Jiang, W., et al. 1992. Cancer Res. 2, 2980.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Xiong, Y., et al. 1992. Genomics 13, 575.
      Xiong, Y., et al. 1992. Cell 71, 504.
      Motokura, T., et al. 1991. Nature 350, 512.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-August-2007 RFH
      ApplicationFlow Cytometry (5 µg/ml)
      Immunofluorescence (5 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
      BackgroundCyclins are a family of proteins implicated in the induction and control of mitosis. They are characterized by their accumulation and dramatic destruction in a cell cycle dependent manner. Cyclins are necessary for cdc/cdk kinase activity and are one of the subunits of the active kinase, in association with cdc2 or one of the cdk family members. Several different major cyclin families have been identified to date. These include the A, B, D and E family of cyclins. The D family of cyclins has been associated with a wide variety of proliferative diseases but their biochemical role is still unknown. The D cyclins appear in early G1, before the B cyclins. The human cyclin D1 gene has been mapped to the 11q13 locus and has been shown to be the PRAD1 gene product. PRAD1 was originally identified as being a clonally rearranged and overexpressed gene linked to the bcl-1 locus in a subset of benign parathyroid tumors. Amplification of cyclin D1 genes has also been demonstrated in human esophageal cancer.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human cyclin D1
      CloneDCS-6
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlA431 or MCF7 cells
      FormLiquid
      FormulationIn 50% glycerol, 50% sodium phosphate buffer.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsDoes not react with cyclins D2 or D3. For immunoblotting, immunohistochemistry, immunoprecipitation and neutralization studies, we recommend Cat. No. CC12. Antibody should be titrated for optimal results in individual systems.
      Storage Protect from light
      Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesInaba, T., et al. 1992. Genomics 13, 565.
      Jiang, W., et al. 1992. Cancer Res. 2, 2980.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Xiong, Y., et al. 1992. Genomics 13, 575.
      Xiong, Y., et al. 1992. Cell 71, 504.
      Motokura, T., et al. 1991. Nature 350, 512.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Application referencesImmunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949. Bartkova, J., et al. 1994. J. Pathol. 172, 237. Lukas, J., et al. 1994. Oncogene 9, 707.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies