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ABE2910 Anti-DNMT3L

Anti-DNMT3L, Cat. No. ABE2910, is a highly specific rabbit polyclonal antibody that targets DNA (cytosine-5)-methyltransferase 3-like and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-DNMT3L MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Dnmt3l UniProt Number: Q9CWR8
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ABE2910
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, IF, WBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2910
      DescriptionAnti-DNMT3L
      Alternate Names
      • DNA (cytosine-5)-methyltransferase 3-like
      Background InformationDNA (cytosine-5)-methyltransferase 3-like (UniProt: Q9CWR8; also known as DNMT3L) is encoded by the Dnmt3l gene (Gene ID: 54427) in murine species. DNMT3L is a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context.
      It is shown to be essential for the function of DNMT3A and DNMT3B. It activates DNMT3A and DNMT3B by binding to their catalytic domain. It acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. DNMT3L recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. In germ cells, it is required for the methylation of imprinted loci together with DNMT3A. In male germ it is essential for the methylation of retrotransposons, which prevents their mobilization. DNMT3A also plays a key role in embryonic stem cells (ESCs) by acting both as a positive and negative regulator of DNA methylation. It promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B. It can also serve as an inhibitor of DNA methylation at the promoter of bivalent genes. It is reported to promote differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. In testicular cells it is first observed in non-dividing prospermatogonia after 12.5 dpc and its level reaches its highest at about the time of birth and then rapidly declines and is non-detectable six days postpartum when most of the prospermatognia are differentiated into dividing spermatogonial stem cells.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DNMT3L, Cat. No. ABE2910, is a highly specific rabbit polyclonal antibody that targets DNA (cytosine-5)-methyltransferase 3-like and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37).

      Immunofluorescence Analysis: A representative lot detected Dnmt3l in mouse testes (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37) and XY germ cells at E15.5 (Kato, Y., et. al. (2016). Nat Commun. 7:11272).

      Immunocytochemistry Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37).
      Biological Information
      ImmunogenHis-tagged full length (421 aa) murine recombinant DNA (cytosine-5)-methyltransferase 3-like (DNMT3L).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Dnmt3l in murine cells.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dnmt3l
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~50 kDa observed; 47.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse embryonic stem cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Dnmt3l in 10 µg of mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE2910 04054839328848