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MABC1594-25UL Anti-DR6 (CD358) Antibody, clone 6B6

Anti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting.

MABC1594-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1594-25UL
      DescriptionAnti-DR6 (CD358) Antibody, clone 6B6
      Alternate Names
      • Tumor necrosis factor receptor superfamily member 21
      • Death receptor 6
      • CD358
      Background InformationTumor necrosis factor receptor superfamily member 21 (UniProt: Q9EPU5; also known as Death receptor 6, DR6, CD358) is encoded by the Tnfrsf21 (also known as Dr6) gene (Gene ID: 94185) in murine species. DR6 is a single-pass type I membrane glycoprotein that is an orphan member of the TNF receptor superfamily. It controls cell death and differentiation in a cell autonomous manner in different cell types. It is ubiquitous expressed and higher expression has been observed in adult spleen, thymus, testis, prostate, ovary, small intestine, colon, brain, lung and kidney tissue. It is also detected in fetal brain, liver and lung. DR6 is expressed at low levels in proliferating progenitors in the spinal cord, but it is highly expressed by differentiating neurons within the spinal cord and adjacent dorsal root ganglia. Expressed by developing neurons as they differentiate and enter a pro-apoptotic state. DR6 promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B and also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. It also participates in neuronal apoptosis in response amyloid peptides derived from amyloid precursor protein. DR6 is reported to be a substrate for ADAM10 and -secretase. DR6 is shown to be involved in peripheral nervous system (PNS) myelination and DR6 knockout mice display precocious myelination in the PNS. DR6 is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 42-349), a transmembrane domain (aa 350-370), and a cytoplasmic domain (aa 371-655). It contains four TNFR-Cys repeats and its death domain is localized to amino acids 415-498. (Ref.: Columbo, A., et al. (2018). EMBO J. 37: e97390).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected DR6 (CD358) in Western Blotting applications (Colombo, A., et. al (2018. EMBO J. 37(7)).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Mouse brain tissue lysates from wild type (WT) versus DR6 knock-out (KO) (Courtesy of Dr. Hung-en Hsia, Group of Neuroproteomics (Head: Prof. Dr. Stefan Lichtenthaler), German Center for Neurodegenerative Diseses (DZNE).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to full-length ectodomain region of mouse DR6 with an N‐terminal CD5 signal peptide and a C‐terminal 2XStrepII tag.
      Epitopeextracellular domain
      Clone6B6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 6B6 is a rat monoclonal antibody that detects Death Receptor 6. It targets an epitope within the extracellular domain.
      IsotypeIgG2b
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tnfrsf21
      • Dr6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight71.98 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity confirmation by isotyping test.



      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1594-25UL 04061842414727

      Documentation

      Anti-DR6 (CD358) Antibody, clone 6B6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-DR6 (CD358) Antibody, clone 6B6 Certificates of Analysis

      TitleLot Number
      Anti-DR6 (CD358), clone 6B6 - Q3490923 Q3490923

      References

      Reference overviewPub Med ID
      Non-cell-autonomous function of DR6 in Schwann cell proliferation
      Alessio Colombo 1 , Hung-En Hsia 1 2 , Mengzhe Wang 3 , Peer-Hendrik Kuhn 2 , Monika S Brill 3 , Paolo Canevazzi 4 , Regina Feederle 1 5 6 , Carla Taveggia 4 , Thomas Misgeld 1 3 6 7 , Stefan F Lichtenthaler
      EMBO J  37(7)  e97390  2018

      Show Abstract       		29459438 29459438