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ST1650 Anti-DYRK1A Mouse mAb (7D10)

Anti-DYRK1A, mouse monoclonal, clone 7D10, recognizes the ~80-85 kDa DYRK1A in PC-12, 293, HeLa NE, Raw 264.7, & NIH3T3 cells. It is validated for use in ELISA and Western blotting.

Anti-DYRK1A Mouse mAb (7D10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A

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ST1650
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

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ST1650-100UG
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      Description
      OverviewRecognizes the ~80-85 kDa DYRK1A protein in PC-12, 293, HeLa NE, Raw 264.7, and NIH3T3 cells.
      Catalogue NumberST1650
      Brand Family Calbiochem®
      SynonymsAnti-Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A
      References
      ReferencesGuo, X., et al. 2010. J. Biol. Chem. 285, 13223.
      Guedj, F., et al. 2009. PLoS One. 4, e4606.
      Hamelet, J., et al. 2009. Biochem. Biophys. Res. Commun. 378, 673.
      Lee, Y., et al. 2009. J. Biol. Chem. 284 33343.
      Noll, C., et al. 2009. PLoS One 2009. 4, e7540.
      Scales, T.M., et al. 2009. J. Cell Sci. 122, 2424.
      Aranda, S., et al. 2008. Mol. Cell Biol. 28, 5899.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlPC-12 cells, 293 cells, HeLa NE cells, Raw 264.7 cells, NIH3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 674-763 human DYRK1A, expressed as a GST fusion protein
      Clone7D10
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ST1650-100UG 04055977223798

      Documentation

      Anti-DYRK1A Mouse mAb (7D10) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Guo, X., et al. 2010. J. Biol. Chem. 285, 13223.
      Guedj, F., et al. 2009. PLoS One. 4, e4606.
      Hamelet, J., et al. 2009. Biochem. Biophys. Res. Commun. 378, 673.
      Lee, Y., et al. 2009. J. Biol. Chem. 284 33343.
      Noll, C., et al. 2009. PLoS One 2009. 4, e7540.
      Scales, T.M., et al. 2009. J. Cell Sci. 122, 2424.
      Aranda, S., et al. 2008. Mol. Cell Biol. 28, 5899.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 JLN
      SynonymsAnti-Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~80-85 kDa DYRK1A protein.
      BackgroundThis gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 674-763 human DYRK1A, expressed as a GST fusion protein
      Clone7D10
      IsotypeIgG2b
      Specieshuman, mouse, rat
      Positive controlPC-12 cells, 293 cells, HeLa NE cells, Raw 264.7 cells, NIH3T3 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesGuo, X., et al. 2010. J. Biol. Chem. 285, 13223.
      Guedj, F., et al. 2009. PLoS One. 4, e4606.
      Hamelet, J., et al. 2009. Biochem. Biophys. Res. Commun. 378, 673.
      Lee, Y., et al. 2009. J. Biol. Chem. 284 33343.
      Noll, C., et al. 2009. PLoS One 2009. 4, e7540.
      Scales, T.M., et al. 2009. J. Cell Sci. 122, 2424.
      Aranda, S., et al. 2008. Mol. Cell Biol. 28, 5899.