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MAB1618-AF488 Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate is an antibody against Dynein for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: DYNC1I1, DYNC1I2 UniProt Number: Q29RQ3, Q0III3
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MAB1618-AF488
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Xn, F, Sh, Dr, B, R, MICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMAB1618-AF488
      DescriptionAnti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Cytoplasmic dynein 1 intermediate chain 1
      • Cytoplasmic dynein intermediate chain 1
      • Dynein intermediate chain 1, cytosolic
      • DH IC-1
      • Cytoplasmic dynein 1 intermediate chain 2
      • Cytoplasmic dynein intermediate chain 2
      • Dynein intermediate chain 2, cytosolic
      • DH IC-2
      Background InformationCytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate is an antibody against Dynein for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cytosolic dynein intermediate chains in A431, and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.
      Biological Information
      ImmunogenBovine brain cytoplasmic dynein.
      EpitopeWithin N-terminal 60 amino acids.
      Clone74.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityReacts with all known forms of the 74 kDa intermediate chain (IC) subunit of mammaliam cytoplasmic dynein. The antigenic site has been mapped to the N-terminal 60 amino acids, a region conserved in intermediate chains encoded by both the DYNC1I1 and DYNC1I2 genes.
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Xenopus
      • Fish
      • Sheep
      • Drosophila
      • Bovine
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DYNC1I1
      • DYNC1I2
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~74 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cytosolic dynein intermediate chains in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1618-AF488 04055977333435

      Documentation

      Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Dynein, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate - 3266601 3266601
      Anti-Dynein, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate - 3873555 3873555
      Anti-Dynein, IC, cytosolic, clone 74.1, Alexa Fluor® 488 Conjugate -Q2667548 Q2667548