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ABS2239-25UL Anti-GPAT4

Anti-GPAT4, Cat. No. ABS2239, is a rabbit polyclonal antibody that detects GPAT and is tested for use in Immunoprecipitation and Western Blotting.

ABS2239-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2239-25UL
      DescriptionAnti-GPAT4
      Alternate Names
      • Glycerol-3-phosphate acyltransferase 4
      • EC:2.3.1.15
      • 1-acylglycerol-3-phosphate O-acyltransferase 6
      • 1-AGP acyltransferase 6
      • 1-AGPAT 6
      • Acyl-CoA:glycerol-3-phosphate acyltransferase 4
      • Lysophosphatidic acid acyltransferase zeta
      • LPAAT-zeta
      • Testis spermatogenesis apoptosis-related protein 7
      • TSARG7
      Background InformationGlycerol-3-phosphate acyltransferase 4 (UniProt: Q86UL3; also known as EC:2.3.1.15, 1-acylglycerol-3-phosphate O-acyltransferase 6, 1-AGP acyltransferase 6; 1-AGPAT 6, Acyl-CoA:glycerol-3-phosphate acyltransferase 4, Lysophosphatidic acid acyltransferase zeta, LPAAT-zeta, Testis spermatogenesis apoptosis-related protein 7, TSARG7) is encoded by the GPAT4 (also known as AGPAT6, TSARG7, UNQ551/PRO1108) gene (Gene ID: 137954) in human. GPAT4 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family that plays a role in triglyceride biosynthesis in several tissues. It is a rate-limiting enzyme that converts glycerol-3-phosphate (G3P) to 1-acyl-sn-glycerol-3-phosphate by incorporating an acyl moiety at the sn-1 position of the glycerol backbone. It is shown to be active against both saturated and unsaturated long-chain fatty acyl-CoAs. Its HXXXXD motif (aa 248-253) is reported to be essential for its acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. By increasing triglyceride synthesis, it protects cells against lipotoxicity induced by high levels of saturated fatty acids. (Ref.: Piccolis, M., et al. (2019). Mol. Cell. 74(1); 32-44; Chen, YQ., et al. (2008). J. Biol. Chem. 283(15); 10048-10057).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GPAT4, Cat. No. ABS2239, is a rabbit polyclonal antibody that detects GPAT and is tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested applications

      Immunoprecipitation Analysis: A representative lot Immunoprecipitated Human GPAT4 protein in Immunoprecipitation applications (Zhu, X.G., et al. (2019). Mol Cell. 74(1):45-58).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 25 amino acids from the N-termina half of human Glycerol-3-phosphate acyltransferase 4 (GPAT4).
      EpitopeN-terminal half
      Concentration0.35 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Glycerol-3-phosphate acyltransferase 4 (GPAT4). It targets an epitope within 25 amino acids from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Porcine, Rat, Feline, Monkey, Mouse, Horse, Bovine, Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GPAT4
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~46 kDa observed; 52.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Human GPAT4 protein in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS2239-25UL 04065269135203