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MABT406 Anti-GPVI Antibody, clone 8E9, Capture Antibody

Anti-GPVI Antibody, clone 8E9, Capture Antibody is an antibody against GPVI for use in western blotting & ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: GP6 UniProt Number: Q9HCN6
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MABT406
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISARPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT406
      ReplacesMABT473
      DescriptionAnti-GPVI Antibody, clone 8E9, Capture Antibody
      Alternate Names
      • Platelet glycoprotein VI
      • GPVI
      • Glycoprotein 6
      Background InformationGlycoprotein VI (GPVI), or glycoprotein 6 (GP6), is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. GPVI is a collagen receptor involved in collagen-induced platelet adhesion and activation, and may contribute to arterial and venous thrombus formation. GPVI is a membrane glycoprotein that plays a crucial role in platelet procoagulant activity and subsequent thrombin and fibrin formation. GPVI is expressed in megakaryocytes and platelets. The signaling pathway of GPVI involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. It has been shown that bleeding disorders such as Bleeding Disorder Platelet-Type 11 (BDPLT11), a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen, is caused by defects in GPVI.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GPVI Antibody, clone 8E9, Capture Antibody is an antibody against GPVI for use in western blotting & ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesELISA Assay: A representative lot from an independent laboratory captured recombinant GPVI in a bead-based sandwich immunoassay (Bigalke, B., et al. (2011). Clin Chem. 57(6):898-904.). This antibody should be used in conjunction with Cat. No. MABT418, Anti-GPVI, clone 8A2, Detection Antibody, as an ELISA capture and detection antibody pair.
      Biological Information
      ImmunogenRecombinant protein corresponding to human GPVI.
      Clone8E9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GP6
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~50 kDa observed. The calculated molecular weight of this protein is 37 kDa. However, a recombinant protein was observed at 50-55 kDa in Western Blotting.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in recombinant human GPV1 glycoprotein.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.1 µg of recombinant human GPV1 glycoprotein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT406 04053252955242

      Documentation

      Anti-GPVI Antibody, clone 8E9, Capture Antibody Certificates of Analysis

      TitleLot Number
      Anti-GPVI, clone 8E9, Capture Antibody - Q2383907 Q2383907

      References

      Reference overviewPub Med ID
      Sandwich immunoassay for soluble glycoprotein VI in patients with symptomatic coronary artery disease.
      Bigalke, Boris, et al.
      Clin. Chem., 57: 898-904 (2011)  2011

      Show Abstract       		21474641 21474641

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies