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05-1533 Anti-Gemin3 Antibody, clone 12H12

Anti-Gemin3 Antibody, clone 12H12 is a Mouse Monoclonal Antibody for detection of Gemin3 also known as Component of gems 3, DEAD-box protein DP103 & has been validated in WB.

Anti-Gemin3 Antibody, clone 12H12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: DDX20, DP103, GEMIN3, Gemin-3 UniProt Number: Q9UHI6
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05-1533
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1533
      DescriptionAnti-Gemin3 Antibody, clone 12H12
      Alternate Names
      • Component of gems 3
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
      • DEAD box protein 20
      • DEAD box protein DP 103
      • DEAD-box protein DP103
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
      • SMN-interacting protein
      Background InformationGemin3 is a member of the DEAD box protein family. The members of this family are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Gemin 3 is a component of the survival of motor neurons (SMN) complex and codes for a DEAD box protein with an ATPase activity The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins and likely functions in the assembly, metabolism, and transport of a diverse number of other ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence of ribonucleic acid (RNA) found in small nuclear RNAs. The complex recognizes specific sequences and structural features of small nuclear RNAs and Sm proteins and assembles small nuclear ribonucleoproteins in a stepwise fashion.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Gemin3 Antibody, clone 12H12 is a Mouse Monoclonal Antibody for detection of Gemin3 also known as Component of gems 3, DEAD-box protein DP103 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein corresponding to human Gemin-3.
      EpitopeUnknown
      Clone12H12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Gemin-3.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq].
      Gene Symbol
      • DDX20
      • DP103
      • GEMIN3
      • Gemin-3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.

      SUBUNIT STRUCTURE: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with SMN1 and with several spliceosomal snRNP core Sm proteins, including SNUPN, SNRPB, SNRPD2 and SNRPD3. Interacts with PPP4R2. Interacts with EBV EBNA2 and EBNA3C.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus › gem. Note: Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

      TISSUE SPECIFICITY: Ubiquitous.

      SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX20 subfamily.

      Contains 1 helicase ATP-binding domain.

      Contains 1 helicase C-terminal domain.
      Molecular WeightDetects a band of approximately 105 kDa (Calculated molecular weight: 92 kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected Gemin-3 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      05-1533 04053252744082