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MABS1190 Anti-Glucokinase/GCK Antibody, clone 9G1.2

Anti-GlucokinaseAntibody, clone 9G1.2 is an antibody against GCK for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-Glucokinase/GCK Antibody, clone 9G1.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Glucose/Glycogen Metabolism Gene Symbol: GCK, HHF3, MODY2 UniProt Number: P35557
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MABS1190
100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1190
      DescriptionAnti-Glucokinase/GCK Antibody, clone 9G1.2
      Alternate Names
      • Glucokinase
      • GK
      • Hexokinase-4
      • Hexokinase-D
      • Hexokinase type IV
      • HK IV
      • HK4
      Background InformationGlucokinase (EC 2.7.1.2; UniProt P35557; also known as GK, Hexokinase-4, Hexokinase-D, Hexokinase type IV, HK IV, HK4) is encoded by the GCK (also known as HHF3, MODY2) gene (Gene ID 2645) in human. Glucokinase/GK functions as the body's primary glucose sensor by catalyzing the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, the rate-limiting step of glucose metabolism in human pancreas and liver. GK activity is regulated rapidly in response to the elevated blood glucose after feeding, whereby a change in GK conformation causes a dissociation of the nuclear GK-glucokinase regulatory protein (GKRP) complex, allowing GK translocation to the cytoplasm in an activated state. In mammals, there exist four types of hexokinases, HK1, HK2, and HK3 have high affinity for glucose, while GK (also known as HK4) has low affinity for glucose and its expression is restricted to the pancreas and liver. GCK gene mutations cause Maturity-onset diabetes of the young 2 (MODY2) and Familial hyperinsulinemic hypoglycemia 3 (HHF3).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GlucokinaseAntibody, clone 9G1.2 is an antibody against GCK for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glucokinase/GCK in human liver, colon, and tonsil tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant internal fragment from the C-terminal half of human Glucokinase/GCK.
      EpitopeInternal (C-terminal half).
      Clone9G1.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9G1.2 recognizes an epitope present in all three spliced isoforms of human Glucokinase/GCK reported by UniProt (P35557).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GCK
      • HHF3
      • MODY2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~52 kDa observed. ~52 kDa observed. 52.19 kDa (isoform 1), 52.13 kDa (isoform 2), and 52.04 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glucokinase/GCK in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1190 04055977343861