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ST1000 Anti-Glutathione Peroxidase Mouse mAb (347)

ST1000
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~22.5 kDa glutathione peroxidase protein in HeLa, U937, PC9, and HL-60 cells.
      Catalogue NumberST1000
      Brand Family Calbiochem®
      SynonymsAnti-GPX
      References
      ReferencesSuemizu, H., et al. 1992. Hybridoma 11, 795.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Negative controlMouse liver, kidney, or plasma cells
      Positive controlHeLa, U937, PC9, HL-60, Jurkat, NB4, or HepG2 cells
      PreservativeNone
      Applications
      Application ReferencesELISA Suemizu, H. et al. 1992. Hybridoma 11, 795.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesImmunoblotting (1:100, chemiluminescence)
      Frozen Sections (1-10 µg/ml)
      Immunocytochemistry (see comments)
      ELISA (see application references)
      Application CommentsThis antibody has also been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenHuman red blood cell glutathione peroxidase
      ImmunogenHuman
      Clone347
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ST1000 0

      Documentation

      Anti-Glutathione Peroxidase Mouse mAb (347) Certificates of Analysis

      TitleLot Number
      ST1000

      References

      Reference overview
      Suemizu, H., et al. 1992. Hybridoma 11, 795.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-November-2008 JSW
      SynonymsAnti-GPX
      ApplicationImmunoblotting (1:100, chemiluminescence)
      Frozen Sections (1-10 µg/ml)
      Immunocytochemistry (see comments)
      ELISA (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~22.5 kDa glutathione peroxidase protein.
      BackgroundGPX is a selenoenzyme that catalyzes the reduction of hydroperoxides in the presence of glutathione (GSH), protecting various cellular components from oxidative damage.
      HostMouse
      Immunogen speciesHuman
      ImmunogenHuman red blood cell glutathione peroxidase
      Clone347
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa, U937, PC9, HL-60, Jurkat, NB4, or HepG2 cells
      Negative controlMouse liver, kidney, or plasma cells
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsThis antibody has also been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSuemizu, H., et al. 1992. Hybridoma 11, 795.
      Application referencesELISA Suemizu, H. et al. 1992. Hybridoma 11, 795.