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MAB2616 Anti-Kindlin-1 Antibody, clone 4A5.14

Anti-Kindlin-1 Antibody, clone 4A5.14 is an antibody against Kindlin-1 for use in WB, IC, IH.

Anti-Kindlin-1 Antibody, clone 4A5.14 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: FERMT1, C20orf42, DTGCU2, KIND1, Kindlerin, Kindlin-1, UNC112A, URP1, kinderlin, kindlin-1 UniProt Number: Q9BQL6
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MAB2616
100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2616
      DescriptionAnti-Kindlin-1 Antibody, clone 4A5.14
      Alternate Names
      • Kindlin syndrome protein
      • UNC-112 related protein 1
      • Unc-112-related protein 1
      • chromosome 20 open reading frame 42
      • kindlin 1
      Background InformationThe Kindlin family is a group of focal adhesion proteins that have recently become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. Specifically associated with Kindler syndrome, is the isoform Kindlin-1 which plays a key role in adhering the actin cytoskeleton to the extracellular matrix of basal keratinocytes. Kindlin-1 may also be involved in tumor growth by modulating TGF-beta 1 signaling (Shi, 2007). It is most widely expressed in the brain, kidney, colon, placenta, skeletal muscle, adrenal gland, and prostate.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Kindlin-1 Antibody, clone 4A5.14 is an antibody against Kindlin-1 for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.)

      Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Papachristou, D.J., et al. (2007). Histopathology. 51(4):499–508.)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Kindlin-1.
      EpitopeUnknown
      Clone4A5.14
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody recognizes Kindlin-1.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq].
      Gene Symbol
      • FERMT1
      • C20orf42
      • DTGCU2
      • KIND1
      • Kindlerin
      • Kindlin-1
      • UNC112A
      • URP1
      • kinderlin
      • kindlin-1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in cell adhesion, possibly via its interaction with integrins. May mediate TGF-beta 1 signaling in tumor progression.

      SUBUNIT STRUCTURE: Interacts with the cytoplasmic domain of integrins ITGB1 and ITGB3.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions. Colocalizes with filamentous actin. Upon TGFB1 treatment, it localizes to membrane ruffles.

      TISSUE SPECIFICITY: Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes.

      INDUCTION: By TGF-beta 1.

      DOMAIN: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.

      INVOLVEMENT IN DISEASE: Defects in FERMT1 are the cause of Kindler syndrome [MIM:173650]. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin.

      SEQUENCE SIMILARITIES: Belongs to the kindlin family.

      Contains 1 FERM domain.

      Contains 1 PH domain.
      Molecular Weight~ 70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB2616 04053252726804

      Documentation

      Anti-Kindlin-1 Antibody, clone 4A5.14 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Kindlin-1 Antibody, clone 4A5.14 Certificates of Analysis

      TitleLot Number
      Anti-Kindlin-1, clone 4A5.14 - 2385982 2385982
      Anti-Kindlin-1, clone 4A5.14 - 2424694 2424694
      Anti-Kindlin-1, clone 4A5.14 - 2433366 2433366
      Anti-Kindlin-1, clone 4A5.14 - 1941609 1941609
      Anti-Kindlin-1, clone 4A5.14 - 2279571 2279571
      Anti-Kindlin-1, clone 4A5.14 - 3202272 3202272
      Anti-Kindlin-1, clone 4A5.14 - 3612296 3612296
      Anti-Kindlin-1, clone 4A5.14 - 3911858 3911858
      Anti-Kindlin-1, clone 4A5.14 - 3936361 3936361
      Anti-Kindlin-1, clone 4A5.14 - 4071505 4071505
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