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MABE1879-25UG Anti-Ku70 Antibody, clone N3H10

Anti-Ku70, clone N3H10, Cat. No. MABE1879, is a mouse monoclonal antibody that detects X-ray repair cross-complementing protein 6 (Ku70) and is tested for use in Immunocytochemistry and Western Blotting.

MABE1879-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1879-25UG
      DescriptionAnti-Ku70 Antibody, clone N3H10
      Alternate Names
      • X-ray repair cross-complementing protein 6
      • 5'-deoxyribose-5-phosphate lyase Ku70
      • 5'-dRP lyase Ku70
      • 70 kDa subunit of Ku antigen
      • ATP-dependent DNA helicase 2 subunit 1
      • ATP-dependent DNA helicase II 70 kDa subunit
      • CTC box-binding factor 75 kDa subunit
      • CTC75
      • CTCBF
      • DNA repair protein XRCC6
      • Lupus Ku autoantigen protein p70
      • Thyroid-lupus autoantigen
      • TLAA
      • X-ray repair complementing defective repair in Chinese hamster cells 6
      Background InformationX-ray repair cross-complementing protein 6 (UniProt: P12956; also known as 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6. Lupus Ku autoantigen protein p70, Ku70, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 6) is encoded by the XRCC6 (also known as G22P1) gene (Gene ID: 2547) in human. Ku autoantigen is a heterodimeric protein composed of Ku70 and Ku80. Ku70 is an evolutionarily conserved protein that binds to DNA double-strand break ends and helps repair DNA via the non-homologous end-joining (NHEJ) pathway.
      The Ku70/Ku80 dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Ku70 serves as a cytosolic DNA sensor that induces the production of IFN- 1, a type III IFN, in primary human cell lines after viral infection after translocating from nucleus to cytosol to interact with STING. (Ref.: Fell, VL., and Schild-Poulter, C. (2012). Mol. Cell Biol. 32(1); 76-87; Sui, H., et al. (2017). Sci. Signaling 10(488); eaah5054).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Ku70, clone N3H10, Cat. No. MABE1879, is a mouse monoclonal antibody that detects X-ray repair cross-complementing protein 6 (Ku70) and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Ku70 in Western Blotting applications (Knuth, M.W., et. al. (1990). J Biol Chem. 265(29):17911-20).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified Protein corresponding to Human X-ray repair cross-complementing protein 6 (Ku70).
      EpitopeC-terminal half
      CloneN3H10
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone N3H10 is a mouse monoclonal antibody that detects Human Ku70 protein. It targets an epitope within the C-terminal half.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • XRCC6
      • G22P1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight69.84 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in PC3 cells.

      Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Ku70 in PC3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1879-25UG 04065265324212