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434629 Anti-Lewis b Mouse mAb (T218)

This Anti-Lewis b Mouse mAb (T218) is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Lewis b.

Anti-Lewis b Mouse mAb (T218) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Leb

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434629
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the Lewis b carbohydrate chain in colon adenocarcinoma.
      Catalogue Number434629
      Brand Family Calbiochem®
      SynonymsAnti-Leb
      References
      ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
      Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
      Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
      Product Information
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Positive controlColon adenocarcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (see comments)
      Immunoblotting (see comments)
      Immunohistochemistry (1:10-1:40)
      Application CommentsFor immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
      Biological Information
      Immunogenhuman SK-CO-10 colon cancer cells
      ImmunogenHuman
      CloneT218
      HostMouse
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      434629 0

      Documentation

      Anti-Lewis b Mouse mAb (T218) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Lewis b Mouse mAb (T218) Certificates of Analysis

      TitleLot Number
      434629

      References

      Reference overview
      Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
      Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
      Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-November-2018 JSW
      SynonymsAnti-Leb
      ApplicationELISA (see comments)
      Immunoblotting (see comments)
      Immunohistochemistry (1:10-1:40)
      DescriptionMouse monoclonal antibody. Recognizes the Lewis b carbohydrate chain.
      BackgroundBlood group antigens are a group of molecules formed by sequential addition of saccharides to carbohydrate side chains of lipids and proteins and are detected on erythrocytes, certain epithelial cells and in secretions of some individuals. A, B and H antigens are synthesized by erythrocytes and integrated into their cell membranes. Lewis antigens are not synthesized by erythrocytes but can be absorbed from the plasma onto the surface of red blood cells. Subtle differences in the composition and arrangement of the sugars in the chains are sufficient to generate these distinct specificities. Lewis b may be detected in gastrointestinal and colorectal tumors and pancreatic cancer.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman SK-CO-10 colon cancer cells
      CloneT218
      IsotypeIgM
      Specieshuman
      Positive controlColon adenocarcinoma tissue
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Preservative≤0.1% sodium azide
      CommentsFor immunohistochemistry, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilutions.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
      Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
      Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.