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ST1116 Anti-MAVS Rabbit pAb

Anti-MAVS Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cardif pAb, Anti-VISA pAb, Anti-IPS-1 pAb

ST1116
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~56 kDa MAVS protein in Saos-2 cells.
      Catalogue NumberST1116
      Brand Family Calbiochem®
      SynonymsAnti-Cardif pAb, Anti-VISA pAb, Anti-IPS-1 pAb
      Application Data
      Lane 1: Detection of human MAVS by immunoblotting. Sample: Whole cell lysate (20 µg) from Saos-2 cells. Primary antibody: Anti-MAVS Rabbit pAb (Cat. No. ST1116) (0.1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of MAVS by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from Saos2 cells. Antibody for immunoprecipitation: Anti MAVS Rabbit pAb (Cat No. ST1116) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated MAVS. Primary antibody: Anti-MAVS Rabbit pAb (Cat No. ST1116) (0.1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesSeth, R.B., et al. 2005. Cell 122, 669.
      McWhirter, S.M., et al. 2005. Cell 122, 645.
      Meylan, E., et al. 2005. Nature 437, 1167.
      Kawai, T., et al. 2005. Nat. Immunol. 6, 981.
      Sun, Q., et al. 2006. Immunity 24, 633.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
      Positive controlSaos-2 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (0.1 µg/ml)
      Immunoprecipitation (10 µg/mg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids in middle region of human MAVS
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ST1116 0

      Documentation

      References

      Reference overview
      Seth, R.B., et al. 2005. Cell 122, 669.
      McWhirter, S.M., et al. 2005. Cell 122, 645.
      Meylan, E., et al. 2005. Nature 437, 1167.
      Kawai, T., et al. 2005. Nat. Immunol. 6, 981.
      Sun, Q., et al. 2006. Immunity 24, 633.

      Brochure

      Title
      Biologics 33.2
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 RFH
      SynonymsAnti-Cardif pAb, Anti-VISA pAb, Anti-IPS-1 pAb
      ApplicationImmunoblotting (0.1 µg/ml)
      Immunoprecipitation (10 µg/mg total protein)
      Application Data
      Lane 1: Detection of human MAVS by immunoblotting. Sample: Whole cell lysate (20 µg) from Saos-2 cells. Primary antibody: Anti-MAVS Rabbit pAb (Cat. No. ST1116) (0.1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of MAVS by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from Saos2 cells. Antibody for immunoprecipitation: Anti MAVS Rabbit pAb (Cat No. ST1116) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated MAVS. Primary antibody: Anti-MAVS Rabbit pAb (Cat No. ST1116) (0.1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinty purified rabbit polyclonal antibody. Recognizes the ~56 kDa MAVS protein.
      BackgroundMAVS (mitochondrial antiviral signaling, accession no. NP_065797) is essential for NF-κB and IRF3 activation by RNA viruses. MAVS contains an N-terminal CARD-like domain and a C-terminal transmembrane domain that targets the protein to the mitochondrial membrane. The mitochondrial targeting transmembrane domain is essential for MAVS signaling, implicating a role for mitochondria in innate immunity. Suppression of MAVS expression blocks interferon production and exacerbates viral replication and killing of host cells. Overexpression of MAVS augments interferon production and confers antiviral immunity. MAVS-deficient cells are compromised in viral-dependent induction of interferons and activation of NF-κB. MAVS-deficient mice fail to induce interferons in response to poly(I:C) stimulation.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids in middle region of human MAVS
      IsotypeIgG
      Specieshuman
      Positive controlSaos-2 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7-8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSeth, R.B., et al. 2005. Cell 122, 669.
      McWhirter, S.M., et al. 2005. Cell 122, 645.
      Meylan, E., et al. 2005. Nature 437, 1167.
      Kawai, T., et al. 2005. Nat. Immunol. 6, 981.
      Sun, Q., et al. 2006. Immunity 24, 633.