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MABS1906-25UG Anti-MULT1 Antibody, clone 1D6

Anti-MULT1, clone 1D6, Cat. No. MABS1906, is a rat monoclonal antibody that detects UL16-binding protein-like transcript 1 splicing variant and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry and Immunoprecipitation.

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MABS1906-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS1906-25UG
      DescriptionAnti-MULT1 Antibody, clone 1D6
      Alternate Names
      • MULT-1
      • ULBP1
      • mouse UL16-binding protein-like Transcript 1
      • NKG2DL
      • NKG2D ligand
      Background InformationUL16-binding protein-like transcript 1 splicing variant (UniProt: Q330P3; also known as MULT1, MULT-1, ULBP1) is encoded by the Ulbp1 gene in murine species. MULT-1 is a MHC Class I-like molecule that serves as a high affinity ligand for natural Killer Group 2D (NKG2D) receptor. It is a type I transmembrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form. Its transmembrane domain is localized to amino acids 124 to 146 and its extracellular domain contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. MULT-1 expression is reported to be low or absent in normal adult tissues, however, under conditions of stress and in tumor cells its levels are elevated. Many tumor cells are shown to release soluble NKG2D ligands through proteolytic shedding, alternative splicing, or exosome secretion. In mice MULT-1 is shown to cause NK cell activation and tumor rejection. Mice subjected to cytomegalovirus (MCMV) infection display strong expression of Ulbp1 (Mult1) gene, but surface expression of MULT-1 is nevertheless completely abolished by the virus. Fc receptor fcr-1 is reported to down-regulate MULT-1 levels by interfering with surface MULT-1 and causing its subsequent degradation in lysosomes. This effect of Fcr-1 can be completely abolished by imipramine (Cat. No. I0899), which blocks AP2-mediated clathrin pathway. (Ref.: Lenac, T., et al. (2006). J. Exp. Med. 203(8); 1843 1850; Krmpotic, A., et al. (2005). J. Exp. Med. 201(2): 211-220; Deng, W., et al. (2015). Science. 348(6230); 136-139).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MULT1, clone 1D6, Cat. No. MABS1906, is a rat monoclonal antibody that detects UL16-binding protein-like transcript 1 splicing variant and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry and Immunoprecipitation.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected MULT1 in Immunocytochemistry applications (Lenac, T., et. al. (2006). J Exp Med. 203(8):1843-50; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).

      Flow Cytometry Analysis: A representative lot detected MULT1 in Flow Cytometry applications (Lenac, T., et. al. (2006). J Exp Med. 203(8):1843-50; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated MULT1 in immunoprecipitation applications. (Krmpotic, A., et al. (2005). J. Exp. Med. 201(2);211-220).

      ELISA Analysis: A representative lot detected MULT1 in ELISA applications (Sheppard, S., et. al. (2017). Nat Commun. 8:13930; Krmpotic, A., et. al. (2005). J Exp Med. 201(2):211-20).
      Biological Information
      ImmunogenRecombinant fragment corresponding to 186 amino acids from the extracellular domain of murine UL16-binding protein-like transcript 1 splicing variant (MULT-1).
      Epitopeextracellular domain
      Clone1D6
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 1D6 is a rat monoclonal antibody that detects UL16-binding protein-like transcript 1 splicing variant in mouse.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ulbp1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight27.59 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Splenocytes from BALB/c mouse.

      Flow Cytometry Analysis: 1 µg of this antibody detected MULT1 in one million splenocytes from BALB/c mouse.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1906-25UG 04054839636615

      Documentation