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ABE2864-25UG Anti-Meis1

Anti-Meis1, Cat. No. ABE2864-25UG, is a highly specific rabbit polyclonal antibody, that targets Homeobox protein Meis1 and has been tested in Western Blotting, Chromatin Immunoprecipitation (ChIP) and Immunofluorescence.

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ABE2864-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2864-25UG
      DescriptionAnti-Meis1
      Alternate Names
      • Homebox protein Meis1
      Background InformationHomeobox protein Meis1 (UniProt: Q60954-1 also known as Myeloid ecotropic viral integration site 1 (Meis1) and Meis1-related protein 1) is encoded by the Meis1 gene (Gene ID: 17268) Meis proteins act as transcription factors that are orthologous to the Drosophila homothorax (Hth) protein. Meis protein contain a TALE (three-amino-acid loop extension) sub-class of the homeodomain that binds to DNA. Meis protein directly bind to Pbx proteins and the Meis/Pbx protein complex binds to DNA through respective Meis- and Pbx-consensus binding sites to regulate transcription. The Meis/Pbx complex plays important roles during development of multiple organs. In humans and mice, three homologues Meis1, Meis2 and Meis3 have been identified. Meis1 is expressed at high levels in the lung and at lower levels in the heart and brain. On the other hand, Meis 2 displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Pancreatic beta cells express both Meis1 and 2. Both Meis1 and 2 are expressed at high levels in all stages of embryonic development of mice (day 7 to 17). Deficiency of Meis1 or Meis 2 can lead to embryonic lethality. (Ref.: Machon, O et al. (2015). BMC Dev. Biol. 515:40).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
      Applications
      ApplicationAnti-Meis1, Cat. No. ABE2864-25UG, is a highly specific rabbit polyclonal antibody, that targets Homeobox protein Meis1 and has been tested in Western Blotting, Chromatin Immunoprecipitation (ChIP) and Immunofluorescence.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: 1 µg/mL of this antibody detected Meis1 in 10 µg of K562 cell lysate.
      Immunofluorescence Analysis: A representative lot detected Meis1 in adjacent stage (St) 41 sagittal hindbrain and eye sections (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
      Western Blotting Analysis: A 1:800 dilution from a representative lot detected Meis1 in HEK293T cells transfectd with Meis1a (C ourtesy of Dr. Valeria Azcoitia and Dr. Miguel Torres ).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33).
      Western Blotting Analysis: A representative lot detected Meis1 in proximal, distal, and RA-treated distal blastemas (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding 14 amino acids from the C-terminal region of Meis1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Meis1 in human and murine cells. It targets an epitope within the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Meis1
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected Meis1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE2864-25UG 04054839464720

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