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ABE2865-100UG Anti-Meis1/2

Anti-Meis1/2 Antibody, Cat. No. ABE2865, is a highly specific rabbit polyclonal antibody that targets Meis1/2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, and Western Blotting.

Anti-Meis1/2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Meis1, Meis2, Mrg1, Stra10 UniProt Number: Q60954-1 and P97367-2 (P97367-3)
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ABE2865-100UG
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MChIP, IF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2865-100UG
      DescriptionAnti-Meis1/2
      Alternate Names
      • Homeobox protein Meis1
      • Myeloid ecotropic viral integration site 1
      • Homeobox protein Meis2
      • Meis1-related protein 1
      Background InformationHomeobox protein Meis1 (UniProt: Q60954-1; also known as Myeloid ecotropic viral integration site 1) Homeobox protein Meis2 (UniProt: P97367-2; also known as Meis1-related protein 1) are encoded by the Meis1 and Meis2 (also known as Mrg1, Stra10) genes (Gene ID: 17268 and 17536) in murine species. Meis proteins act as transcription factors that are orthologous to the Drosophila homothorax (Hth) protein. They are expressed dynamically in several organs during embryogenesis and exert potent regulatory activity through their interaction with Hox proteins and other transcription factors. Meis protein contain a TALE (three-amino-acid loop extension) sub-class of the homeodomain that binds to DNA. They directly bind to Pbx proteins and the Meis/Pbx protein complex binds to DNA through respective Meis- and Pbx-consensus binding sites to regulate transcription. The Meis/Pbx complex plays important roles during development of multiple organs. In humans and mice, three homologues Meis1, Meis2 and Meis3 have been identified. Meis1 is expressed at high levels in the lung and at lower levels in the heart and brain. On the other hand, Meis 2 displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Pancreatic beta cells express both Meis1 and 2. Both Meis1 and 2 are expressed at high levels in all stages of embryonic development of mice (day 7 to 17). Meis3 is expressed in pancreatic islets and is reported to control the expression of expression of PDK1, which promotes survival of pancreatic beta-cells. Deficiency of Meis1 or Meis 2 can lead to embryonic lethality. Meis1-deficient mice die around midgestation due to a general failure of definitive hematopoiesis. (Ref.: Machon, O et al. (2015). BMC Dev. Biol. 515:40; Azcoitia, V et al., (2005). Dev. Biol. 280 (2); 307-320).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
      Applications
      ApplicationAnti-Meis1/2 Antibody, Cat. No. ABE2865, is a highly specific rabbit polyclonal antibody that targets Meis1/2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected Meis1/2 in Immunofluorescence applications (Mercader, N., et. al. (2005). Development. 132(18):4131-42; Marcos, S., et. al. (2015). Development. 142(17):3009-20).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1/2 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33; Marcos, S., et. al. (2015). Development. 142(17):3009-20).

      Western Blotting Analysis: A representative lot detected Meis1/2 in Western Blotting applications (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of murine Meis1a, Meis2a, and 13 amino acids from Meis2b isoforms.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Meis1/2. It targets an epitope with in 14 amino acids from the C-terminal region.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Salamander, Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Meis1
      • Meis2
      • Mrg1
      • Stra10
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~51 kDa observed; 43.00, 43.79, and 43.06 kDa for Meis1a, Meis2a, and Meis2b, respectively. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cells transfected with pCMVTAG2-Flag-Meis2a.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Meis1/2 in HEK293T cells transfected with pCMVTAG2-Flag-Meis2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE2865-100UG 04054839551017