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NB21 Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NSF

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NB21
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~76 kDa NSF-1 protein in human fibroblasts, MDBK, and PC12 cells.
      Catalogue NumberNB21
      Brand Family Calbiochem®
      SynonymsAnti-NSF
      References
      ReferencesSutton, R.B., et al. 1998. Nature 395, 347.
      Lemons, P.P., et al. 1997. Blood 90, 1490.
      Edelmann, L., et al. 1995. EMBO J. 14, 224.
      Südhof, T.C. 1995. Nature 375, 645.
      Whiteheart, S.W., et al. 1994. J. Cell Biol. 126, 945.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
      Positive controlHuman or rat fibroblasts, MDBK cells, or PC12 cells
      Preservative3 mM sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (1:2000)
      Immunoblotting (1:2000)
      Immunoprecipitation (1:500)
      Application CommentsThis antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human N-ethylmaleimide-senstive factor
      ImmunogenHuman
      CloneNSF-1
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      NB21 0

      Documentation

      Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1) Certificates of Analysis

      TitleLot Number
      NB21

      References

      Reference overview
      Sutton, R.B., et al. 1998. Nature 395, 347.
      Lemons, P.P., et al. 1997. Blood 90, 1490.
      Edelmann, L., et al. 1995. EMBO J. 14, 224.
      Südhof, T.C. 1995. Nature 375, 645.
      Whiteheart, S.W., et al. 1994. J. Cell Biol. 126, 945.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      SynonymsAnti-NSF
      ApplicationELISA (1:2000)
      Immunoblotting (1:2000)
      Immunoprecipitation (1:500)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~76 kDa NSF-1 protein.
      BackgroundSynaptic vesicle proteins can be divided into one of two classes; transport proteins involved in neurotransmitter uptake, and trafficking proteins which perform functions in vesicle docking, fusion, and budding. For example, synaptophysin may function in the process of neurotransmitter release, while synaptobrevin (VAMP) is believed to play a role in synaptic vesicle docking and exocytotic membrane fusion. Synaptobrevin has also been shown to bind synaptophysin with high specificity. Synapsins are highly abundant vesicle membrane proteins that are believed to regulate neurotransmitter release through phosphorylation-dependent interactions with cytoskeletal elements. Other proteins involved in vesicle exocytosis include syntaxin 1 and SNAP-25. In conjunction with synaptobrevin, these proteins form the SNARE (SNAP receptor) complex, which recruits other cytosolic factors including SNAP proteins (soluble NSF attachment proteins) and NSF (N-ethylmaleimide-sensitive factor). NSF acts in concert with SNAPs and SNAREs for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicle-to-plasma membrane transport. In its functional form, NSF is a homotrimer of 76 kDa subunits. Each subunit contains an N-terminal domain and two homologous ATP binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. SNAPs α and γ then bind to this docking complex and mediate the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human N-ethylmaleimide-senstive factor
      CloneNSF-1
      IsotypeIgG
      Specieshuman, rat
      Positive controlHuman or rat fibroblasts, MDBK cells, or PC12 cells
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative3 mM sodium azide
      CommentsThis antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSutton, R.B., et al. 1998. Nature 395, 347.
      Lemons, P.P., et al. 1997. Blood 90, 1490.
      Edelmann, L., et al. 1995. EMBO J. 14, 224.
      Südhof, T.C. 1995. Nature 375, 645.
      Whiteheart, S.W., et al. 1994. J. Cell Biol. 126, 945.