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MABN1787 Anti-NAV1 Antibody, clone 7F12 (KT124)

Anti-NAV1 Antibody, clone 7F12 (KT124) is an antibody against NAV1 for use in Immunocytochemistry, Western Blotting.

Anti-NAV1 Antibody, clone 7F12 (KT124) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: NAV1, KIAA1151, KIAA1213, POMFIL3, STEERIN1 UniProt Number: Q8NEY1
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MABN1787
100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1787
      DescriptionAnti-NAV1 Antibody, clone 7F12 (KT124)
      Alternate Names
      • Neuron navigator 1
      • Pore membrane and/or filament-interacting-like protein 3
      • Steerin-1
      • Unc-53 homolog 1
      • unc53H1
      • NAV1
      Background InformationNeuron navigator 1 (UniProt Q8NEY1; also known as Pore membrane and/or filament-interacting-like protein 3, Steerin-1, Unc-53 homolog 1, unc53H1) is encoded by the NAV1 (also known as KIAA1151, KIAA1213, POMFIL3, STEERIN1, UNC53H1) gene (Gene ID 89796) in human. NAV1 is a member of the family of proteins called navigators that play a role in mediating neurite outgrowth. NAV1 is shown to interact with the guanine nucleotide exchange factor (GEF) TRIO, whereby it enhances the affinity of TRIO for Rho family GTPases Rac1 and RhoG. The plus-end-tracking protein (+TIP) EB1 at the ends of growing microtubules recruits TRIO, facilitating the formation and localization of functional NAV1-TRIO complexes, which in turn regulates neurite outgrowth by selectively activating Rac1 at the growth cone.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in PBS and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NAV1 Antibody, clone 7F12 (KT124) is an antibody against NAV1 for use in Immunocytochemistry, Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected NAV1 in 10 µg of Murine NIE-115 neuroblastoma cell lysate.
      Immunocytochemistry Analysis: 10.0 µg/mL from a representative lot detected NAV1 in Neuro-2a and NIE-115 cells.
      Western Blotting Analysis: A representative lot detected endogenous NAV1 protein in murine neuroblastoma N1E-115 cell lysate, as well as exogenously expressed NAV1, but not NAV2 or NAV3, GFP fusion protein in lysates from transfected HEK293T cells (van Haren, J., et al. (2014). Curr. Biol. 24(15):1778-1785).
      Immunocytochemistry Analysis: A representative lot detected NAV1 accumulation in cellular extensions of primary murine hippocampal neurons and murine neuroblastoma N1E-115 cells (van Haren, J., et al. (2014). Curr. Biol. 24(15):1778-1785).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to the Internal region of human NAV1.
      EpitopeInternal region
      CloneKT124(7F12)
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityExpected to react with all seven spliced isoforms of murine NAV1 (UniProt Q8NEY1-1 through Q8NEY1-7), but not NAV2 or NAV3.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NAV1
      • KIAA1151
      • KIAA1213
      • POMFIL3
      • STEERIN1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~240/150 kDa observed. 202.5 kDa (isoform 1), 201.5 kDa (isoform 2), 202.1 kDa (isoform 3), 196.0 kDa (isoform 4), 160.8 kDa (isoform 5), 126.2 kDa (isoform 6), 195.0 kDa (isoform 7) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in murine Neuro-2a (N2a) neuroblastoma cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected NAV1 in 10 µg of murine Neuro-2a (N2a) neuroblastoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN1787 04055977297690