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PC344 Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb

Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC344
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      MRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~70 kDa netrin-1 protein in embryonic mouse brain tissue.
      Catalogue NumberPC344
      Brand Family Calbiochem®
      Application Data
      Detection of mouse netrin-1 by immunoblotting. Samples: Whole tissue extract from mouse brain harvested at embryonic days 14 (E14) and 16 (E16), postnatal day 1 (P1), and adult (A). Primary antibody: Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb (Cat. No. PC344) (1 μg/ml). Detection: chemiluminescence.
      References
      ReferencesVarela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev. 11, 545.
      Harris, R., et al. 1996. Neuron 17, 217.
      Keino-Masu, K., et al. 1996. Cell 87, 175.
      Mitchell, K.J., et al. 1996. Neuron 17, 203.
      Serafini, T., et al. 1996. Cell 87, 1001.
      Tessier-Lavigne, M. and Goodman, C.S. 1996. Science 274, 1123.
      Kennedy, T.E., et al. 1994. Cell 78, 425.
      Serafini, T., et al. 1994. Cell 78, 409.
      Ishii, N., et al. 1992. Neuron 9, 873.
      Hedgecock, E.M., et al. 1990. Neuron 4, 61.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlMouse brain tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml)
      Frozen Sections (not recommended)
      Application CommentsImmunoblotting was performed using brain tissue from various developmental stages. Netrin-1 protein is enriched in embryonic neural tissue. Antibody should be titrated for optimal results in individual systems. For immunohistochemistry applications, Anti-Netrin-1 (Ab-2) Rabbit pAb is also available (Cat. No. PC364-100 µg).
      Biological Information
      Immunogena synthetic peptide (LGNAEDSPDQSG) corresponding to amino acids 557-568 of mouse netrin-1, conjugated to KLH
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PC344 0

      Documentation

      Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC344

      References

      Reference overview
      Varela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev. 11, 545.
      Harris, R., et al. 1996. Neuron 17, 217.
      Keino-Masu, K., et al. 1996. Cell 87, 175.
      Mitchell, K.J., et al. 1996. Neuron 17, 203.
      Serafini, T., et al. 1996. Cell 87, 1001.
      Tessier-Lavigne, M. and Goodman, C.S. 1996. Science 274, 1123.
      Kennedy, T.E., et al. 1994. Cell 78, 425.
      Serafini, T., et al. 1994. Cell 78, 409.
      Ishii, N., et al. 1992. Neuron 9, 873.
      Hedgecock, E.M., et al. 1990. Neuron 4, 61.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Frozen Sections (not recommended)
      Application Data
      Detection of mouse netrin-1 by immunoblotting. Samples: Whole tissue extract from mouse brain harvested at embryonic days 14 (E14) and 16 (E16), postnatal day 1 (P1), and adult (A). Primary antibody: Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb (Cat. No. PC344) (1 μg/ml). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~60 kDa netrin-1 protein.
      BackgroundIn the developing nervous system, axon guidance and the establishment of appropriate neuronal connections involves a number of chemoattractant and chemorepellent molecules. Netrins are related to the extracellular matrix protein laminin and comprise a family of secreted molecules capable of guiding the growth cone. The first netrin family member to be characterized, UNC-6 from C. elegans, was initially identified from the gene mutation which lead to defects in circumferential axon migration in the nematode. In contrast, the identification of the first vertebrate netrins, designated netrin-1 and netrin-2, resulted from a biochemical rather than genetic approach. Netrin homologs have subsequently been identified from a number of species. Results from several studies suggest a role for netrin-1 in mediating chemotropic and outgrowth-promoting activities of floor plate cells to guide commissural axons towards the ventral midline. DCC (Deleted in Colorectal Cancer) is believed to function as a component of a receptor complex which mediates the effects of netrin-1 on commissural axons and helps coordinate the navigation of axons to establish precise patterns of neural connectivity. In addition to the netrins and their receptors, a number of other diffusable or membrane-associated ligand families and their receptors have been implicated in axon guidance, including the semaphorins, ephrins, and cell adhesion molecules.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide (LGNAEDSPDQSG) corresponding to amino acids 557-568 of mouse netrin-1, conjugated to KLH
      IsotypeIgG
      Speciesmouse
      Positive controlMouse brain tissue
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunoblotting was performed using brain tissue from various developmental stages. Netrin-1 protein is enriched in embryonic neural tissue. Antibody should be titrated for optimal results in individual systems. For immunohistochemistry applications, Anti-Netrin-1 (Ab-2) Rabbit pAb is also available (Cat. No. PC364-100 µg).
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesVarela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev. 11, 545.
      Harris, R., et al. 1996. Neuron 17, 217.
      Keino-Masu, K., et al. 1996. Cell 87, 175.
      Mitchell, K.J., et al. 1996. Neuron 17, 203.
      Serafini, T., et al. 1996. Cell 87, 1001.
      Tessier-Lavigne, M. and Goodman, C.S. 1996. Science 274, 1123.
      Kennedy, T.E., et al. 1994. Cell 78, 425.
      Serafini, T., et al. 1994. Cell 78, 409.
      Ishii, N., et al. 1992. Neuron 9, 873.
      Hedgecock, E.M., et al. 1990. Neuron 4, 61.