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AB5894 Anti-P2X2 Receptor Antibody

Anti-P2X2 Receptor Antibody is an antibody against P2X2 Receptor for use in IC, IH & WB.

Anti-P2X2 Receptor Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: P2RX2, MGC129601, P2X2 UniProt Number: Q9UBL9
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AB5894
50 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RICC, IHC, WBGpSerumPolyclonal Antibody
      Description
      Catalogue NumberAB5894
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-P2X2 Receptor Antibody
      References
      Product Information
      FormatSerum
      PresentationSerum. Liquid. Contains 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-P2X2 Receptor Antibody is an antibody against P2X2 Receptor for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunoblotting: 1:500

      Immunohistochemistry: 1:500.

      Immunocytochemistry: 1:500.

      Optimal working dilutions must be determined by end user.

      APPLICATION NOTES FOR AB5894

      IMMUNOHISTOCHEMISTRY

      Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5894 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

      IMMUNOCYTOCHEMISTRYP

      2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above

      WESTERN BLOTTING

      Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with AB5894 diluted 1:500 with same buffer overnight at 2-8°C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence.
      Biological Information
      ImmunogenA 13 amino acid peptide corresponding to amino acids 460-472 from the carboxy-terminus of the rat P2X2 Receptor protein.

      Control peptide: Catalog Number AG354
      HostGuinea Pig
      SpecificityP2X2 Receptor.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for this gene.
      Gene Symbol
      • P2RX2
      • MGC129601
      • P2X2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBL9 # Binding of this ligand gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle.
      SIZE: 471 amino acids; 51754 Da
      SUBUNIT: Homo- or heteropolymers.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: Q9UBL9 ## Belongs to the P2X receptor family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB5894 04053252590771

      Documentation

      Anti-P2X2 Receptor Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-P2X2 Receptor Antibody Certificates of Analysis

      TitleLot Number
      GUINEA PIG ANTI-P2X2 RECEPTOR - 3307748 3307748
      GUINEA PIG ANTI-P2X2 RECEPTOR - 3476996 3476996
      GUINEA PIG ANTI-P2X2 RECEPTOR - 3506447 3506447
      GUINEA PIG ANTI-P2X2 RECEPTOR - 3574389 3574389
      GUINEA PIG ANTI-P2X2 RECEPTOR - 3704212 3704212
      GUINEA PIG ANTI-P2X2 RECEPTOR POLYCLONAL ANTIBODY 2928734
      GUINEA PIG ANTI-P2X2 RECEPTOR POLYCLONAL ANTIBODY 2984197
      GUINEA PIG ANTI-P2X2 RECEPTOR POLYCLONAL ANTIBODY 3068549
      GUINEA PIG ANTI-P2X2 RECEPTOR POLYCLONAL ANTIBODY 2857692
      GUINEA PIG ANTI-P2X2 RECEPTOR POLYCLONAL ANTIBODY - 2050220 2050220
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      Data Sheet

      Title
      GUINEA PIG ANTI-P2X2 RECEPTOR