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ST1537 Anti-PCSK1 Mouse mAb

Anti-PCSK1 Mouse mAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Proprotein Convertase Subtilisin/Kexin 1

ST1537
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~80 kDa PCSK1 protein in HeLa, PC12, Raw 264.7, and NIH3T3 cells and human small intestine tissue.
      Catalogue NumberST1537
      Brand Family Calbiochem®
      SynonymsAnti-Proprotein Convertase Subtilisin/Kexin 1
      Application Data
      Detection of human PCSK1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of rat PCSK1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PCSK1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human PCSK1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (30 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, PC12 cells, Raw 264.7 cells, NIH3T3 cells, or Human small intestine tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 652-754 of human PCSK1, expressed as a GST fusion protein
      HostMouse
      IsotypeIgG₃
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ST1537 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-Proprotein Convertase Subtilisin/Kexin 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human PCSK1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of rat PCSK1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PCSK1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (3 µg/ml). Detection: colorimetric (DAB).

      Detection of human PCSK1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (30 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~80 kDa PCSK1 protein.
      BackgroundThe members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 652-754 of human PCSK1, expressed as a GST fusion protein
      IsotypeIgG₃
      Specieshuman, mouse, rat
      Positive controlHeLa cells, PC12 cells, Raw 264.7 cells, NIH3T3 cells, or Human small intestine tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review