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AB3349 Anti-PRMT5 Antibody, CT

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AB3349
400 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3349
      ReplacesMABE551
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PRMT5 Antibody, CT
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: HL60 cell lysates.
      PresentationPurified immunoglobulin in PBS containing 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PRMT5 Antibody, C-terminus is a high quality Rabbit Polyclonal Antibody for the detection of PRMT5 & has been validated in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern Blot: 1:100-1:500. Reacts with a band of approximately 70 kDa on whole HL60 cell lysate. Additional bands may be present depending on sample used.

      ELISA (direct): 1:1,000

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from C-terminus of human PRMT5.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityReacts with PRMT5.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRMT5
      • SKB1
      • HRMT1L5
      • IBP72
      • SKB1Hs
      • JBP1
      • EC 2.1.1.125
      • EC 2.1.1.-
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14744 # Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.
      SIZE: 637 amino acids; 72684 Da
      SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Disulfide bonds and non-covalent association mediate homooligomers formation.
      SIMILARITY: SwissProt: O14744 ## Belongs to the protein arginine N-methyltransferase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size400 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB3349 04053252622649

      Documentation

      Anti-PRMT5 Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PRMT5 Antibody, CT Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-PRMT5 -2622675 2622675
      RABBIT ANTI-PRMT5 C-TERMINAL - 3561548 3561548
      RABBIT ANTI-PRMT5 C-TERMINAL - 3666804 3666804
      RABBIT ANTI-PRMT5 C-TERMINAL - 3859600 3859600
      RABBIT ANTI-PRMT5 C-TERMINAL - 4120673 4120673
      RABBIT ANTI-PRMT5 C-TERMINAL POLYCLONAL ANTIBODY 2979901

      References

      Reference overviewPub Med ID
      Establishment of a versatile seminoma model indicates cellular plasticity of germ cell tumor cells.
      Daniel Nettersheim,Birgit Westernströer,Natalie Haas,Anke Leinhaas,Oliver Brüstle,Stefan Schlatt,Hubert Schorle
      Genes, chromosomes & cancer  51  2012

      Show Abstract
      22489004 22489004
      Exposure on cell surface and extensive arginine methylation of ewing sarcoma (EWS) protein.
      Belyanskaya, L L, et al.
      J. Biol. Chem., 276: 18681-7 (2001)  2001

      11278906 11278906
      Arginine methylation of a mitochondrial guide RNA binding protein from Trypanosoma brucei.
      Pelletier, M, et al.
      Mol. Biochem. Parasitol., 118: 49-59 (2001)  2001

      Show Abstract
      11704273 11704273
      Crystal structure of the conserved core of protein arginine methyltransferase PRMT3.
      Zhang, X, et al.
      EMBO J., 19: 3509-19 (2000)  2000

      Show Abstract
      10899106 10899106
      PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain.
      Frankel, A and Clarke, S
      J. Biol. Chem., 275: 32974-82 (2000)  2000

      Show Abstract
      10931850 10931850

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies