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MABN2300-25UG Anti-PSMA/FOLH1 Antibody, clone 3F11

Anti-PSMA/FOLH1, clone 3F11, Cat. No. MABN2300, is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA) and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

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MABN2300-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2300-25UG
      DescriptionAnti-PSMA/FOLH1 Antibody, clone 3F11
      Alternate Names
      • Glutamate carboxypeptidase 2
      • EC:3.4.17.21
      • Cell growth-inhibiting gene 27 protein
      • Folate hydrolase 1
      • Folylpoly-gamma-glutamate carboxypeptidase
      • FGCP
      • Glutamate carboxypeptidase II
      • GCPII
      • Membrane glutamate carboxypeptidase
      • N-acetylated-alpha-linked acidic dipeptidase I
      • NAALADase I
      • Prostate-specific membrane antigen
      • PSM
      • Pteroylpoly-gamma-glutamate carboxypeptidase
      Background InformationGlutamate carboxypeptidase 2 (UniProt: Q04609; also known as EC:3.4.17.21, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase) is encoded by the FOLH1 (also known as FOLH, NAALADI, PSM, PSMA, GIG27) gene (Gene ID: 2346) in human. PSMA is a single-pass type II membrane protein that has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase activities. It exhibits a preference for tri-alpha-glutamate peptides. Its expression pattern is restricted mainly to the healthy prostate secretory-acinar epithelium and the plasma membrane of epithelial prostate carcinoma. In the brain, it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG) and release of glutamate. Clone 3F11 is shown to be highly specific for PSMA and does not exhibit any cross-reactivity with unrelated proteins. It binds to a linear epitope (aa 226-243) within the extracellular domain and is reported to be suitable for the detection of PSMA orthologs from some other species when denatured form of PSMA is used. (Ref.: Vahidfar, N., et al., (2019). J. Radioanal. Nucl. Chem. 322:237 248; Novakova, Z., et al. (2017). The Prostate 77(7); 749-764).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PSMA/FOLH1, clone 3F11, Cat. No. MABN2300, is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA) and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • ELISA
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected PSMA in Immunofluorescence applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

      ELISA Analysis: A representative lot detected PSMA in ELISA applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

      Immunohistochemistry (Paraffin) Analysis: A 1:80,000 dilution from a representative lot detected PSMA in human prostate tissues (Courtesy of Barinka, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic and J. Hrudka, University Hospital Vinohrady, Prague, Czech Republic).

      Immunohistochemistry Applications: A representative lot detected PSMA in Immunohistochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

      Immunocytochemistry Analysis: A representative lot detected PSMA in Immunocytochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

      Western Blotting Analysis: A representative lot detected PSMA in Western Blotting applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human Prostate-specific membrane antigen (PSMA).
      EpitopeExtracellular domain
      Clone3F11
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3F11 is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA). It targets an epitope within 18 amino acids from the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Porcine
      • Mouse
      • Human
      • Rat
      • Canine
      Species Reactivity NoteHuman, Canine, Mouse, Porcine, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • FOLH1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~120 kDa observed; 84.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in LNCap cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PSMA in LNCap cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2300-25UG 04065267404158