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PC10L Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb

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PC10L
  
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Overview

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~21 kDa H-, K-, and N-Ras proteins containing aspartic acid at position 12.

Catalogue NumberPC10L
Brand Family Calbiochem®
References
ReferencesBos, J.L., et al. 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1990 Nature 287, 686.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.
Negative controlSW480 cells
Positive controlHS578T cells or purified recombinant K-Ras Asp¹² protein
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml)
Application CommentsDoes not react with rasGly-12 or rasVal-12 p21's. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.
Global Trade Item Number
Catalog Number GTIN
PC10L 0