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CB1016 Anti-Paxillin Mouse mAb (M107)

Anti-Paxillin Mouse mAb (M107) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CB1016
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~68 kDa paxillin protein in A431 cells.
      Catalogue NumberCB1016
      Brand Family Calbiochem®
      Application Data
      Detection of human paxillin by immunoblotting. Samples: Whole cell lysate from A431 cells left untreated (-) or treated with EGF (+; 100 ng/ml for 5 min). Primary antibodies: Anti-Paxillin Mouse mAb (M107) (1:1000) (panel A) and Anti-Paxillin (pSer178) (panel B). Detection: chemiluminescence.
      References
      ReferencesHuang, C., et al. 2004. Cell Cycle 3, 4.
      Woodrow, M.A., et al. 2003. Exp. Cell Res. 287, 325.
      Huang, C., et al. 2003. Nature 424, 219.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
      Preservative≤ 0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesELISA (1:2000)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:500)
      Immunoprecipitation (1-5 µl)
      Application CommentsFor immunoblotting the antibody should be diluted in PBS, 5% BSA, 0.04% Tween®-20 detergent for 1 h at room temperature. Specificity for total paxillin was confirmed by comparison to a phospho-specific anti-paxillin antibody (see application data). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human paxillin
      ImmunogenHuman
      CloneM107
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CB1016 0

      Documentation

      Anti-Paxillin Mouse mAb (M107) Certificates of Analysis

      TitleLot Number
      CB1016

      References

      Reference overview
      Huang, C., et al. 2004. Cell Cycle 3, 4.
      Woodrow, M.A., et al. 2003. Exp. Cell Res. 287, 325.
      Huang, C., et al. 2003. Nature 424, 219.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationELISA (1:2000)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:500)
      Immunoprecipitation (1-5 µl)
      Application Data
      Detection of human paxillin by immunoblotting. Samples: Whole cell lysate from A431 cells left untreated (-) or treated with EGF (+; 100 ng/ml for 5 min). Primary antibodies: Anti-Paxillin Mouse mAb (M107) (1:1000) (panel A) and Anti-Paxillin (pSer178) (panel B). Detection: chemiluminescence.
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~68 kDa paxillin protein.
      BackgroundPaxillin is a focal adhesion protein that is involved in the formation of focal adhesions during cell adhesion and migration. The native protein contains LD motifs, LIM domains, and SH3-/SH2-binding domains that facilitate a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin at Ser178 by JNK1 and cdc2 occurs in response to growth factor activation and fibronectin interaction. Mutation of Ser178 to alanine results in a decrease in the migration of keratinocytes and epithelial cells, suggesting that phosphorylation at this site is an important factor in cell migration.
      HostMouse
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human paxillin
      CloneM107
      IsotypeIgG₁
      Specieschicken, human, mouse, rat
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
      Preservative≤ 0.1% sodium azide
      CommentsFor immunoblotting the antibody should be diluted in PBS, 5% BSA, 0.04% Tween®-20 detergent for 1 h at room temperature. Specificity for total paxillin was confirmed by comparison to a phospho-specific anti-paxillin antibody (see application data). Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesHuang, C., et al. 2004. Cell Cycle 3, 4.
      Woodrow, M.A., et al. 2003. Exp. Cell Res. 287, 325.
      Huang, C., et al. 2003. Nature 424, 219.