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MABE341-AF488 Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody

This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
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MABE341-AF488
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMABE341-AF488
      DescriptionAnti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody
      Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      This product is a conjugated version of Cat. No. MABE341.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenPuromycin-incorporated neosynthesized proteins at multiple molecular weights
      Clone17H1
      Concentration0.5 mg/mL
      HostRat
      SpecificityThis antibody detects Puromycin-incorporated neosynthesized proteins.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightRefer to Cat. No. MABE341 for observed molecular weight information.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE341-AF488 04053252973895

      Documentation

      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody Certificates of Analysis

      TitleLot Number
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - Q2425063 Q2425063
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 3691372 3691372
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 3855700 3855700
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 3991432 3991432
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate - 4127491 4127491
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Monoclonal Antibody 3093931
      Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate_2822507 2822507

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies