Millipore Sigma Vibrant Logo

PC130 Anti-Rad51 (Ab-1) Rabbit pAb

Overview

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
PC130-100UL
Retrieving availability...
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Plastic ampoule 100 ul
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      OverviewRecognizes the ~43 kDa Rad51 protein in ovarian tissue.
      Catalogue NumberPC130
      Brand Family Calbiochem®
      Application Data
      Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

      Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

      Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
      References
      ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
      Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
      Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Sharan, S.K., et al. 1997. Nature 386, 304.
      Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
      Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
      Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
      Tashiro, S., et al. 1996. Oncogene 12, 2165.
      Ashley, T., et al. 1995. Chromosoma 104, 19.
      Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
      Positive controlA431 cells, HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:2500)
      Immunoprecipitation (40 µl/500µg total protein from lysate)
      Immunocytochemistry (1:500)
      Application CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
      Biological Information
      Immunogenrecombinant, human Rad51
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalog Number GTIN
      PC130-100UL 07790788053949