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MAB994-I-25UG Anti-Reovirus Type 3 sigma 1 hemagglutinin Antibody, clone 9BG5

Anti-Reovirus Type 3 sigma 1 hemagglutinin, clone 9BG5, Cat. No. MAB994-I, is a mouse monoclonal antibody that detects sigma 1 hemagglutinin of Reovirus type 3 and has been tested for use in Flow Cytometry, Radioimmunoassay, Immunofluorescence, and neutralizing applications.

MAB994-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB994-I-25UG
      DescriptionAnti-Reovirus Type 3 sigma 1 hemagglutinin Antibody, clone 9BG5
      Alternate Names
      • T3 sigma 1
      • Reovirus T3 sigma 1
      Background InformationMammalian reoviruses are nonenveloped viruses with a segmented, double-stranded RNA genome. Although these viruses can infect most mammalian species, they are usually asymptomatic in human. In neonatal SCID NOD mice they can cause lethal infection. Three major reovirus serotypes have been described that are differentiated by the capacity of antisera to neutralize viral infectivity and block hemagglutination. Type 1 and 3 sigma 1 contain receptor-binding domains in both the tail and head regions and in type 3 sigma 1 tail is shown to bind alpha-linked sialic acid. Sigma 1 is reported to engage glycan receptors and junctional adhesion molecule-A (JAM-A) and undergoes a conformational change during the proteolytic disassembly of virions to infectious subvirion particles (ISVPs) that accompany cell entry. Type 3 reovirus is shown to spread to the central nervous system and causes lethal encephalitis in murine models. Reovirus can also serve an oncolytic function and can hijack the activated Ras signaling pathway of host tumor cells. It can induce apoptosis in tumor cells through both extrinsic and intrinsic pathways. In this process viral uncoating is reported to be an essential factor. Clone 9BG5 is shown to efficiently block HA by strain type 1 Lang (T1L) and ISVPs. (Ref.: Yang WQ et al. (2004). Clin. Cancer Res. 10(24); 8561-8576; Tyler, KL., et al. (2004). J. Infect. Dis. 189(9); 1664-1675; Dietrich, MH., et al. (2017). J. Virology 91(4); e01621-16).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Reovirus Type 3 sigma 1 hemagglutinin, clone 9BG5, Cat. No. MAB994-I, is a mouse monoclonal antibody that detects sigma 1 hemagglutinin of Reovirus type 3 and has been tested for use in Flow Cytometry, Radioimmunoassay, Immunofluorescence, and neutralizing applications.
      Key Applications
      • Flow Cytometry
      • Radioimmunoassay
      • Immunofluorescence
      • Neutralizing
      Application NotesImmunofluorescence Analysis: A representative lot detected Reovirus Type 3 sigma 1 hemagglutinin in Immunofluorescence applications (Barton, E.S., et. al. (2001). J Biol Chem. 276(3):2200-2211).

      Radioimmunoassay Analysis: A representative lot detected Reovirus Type 3 sigma 1 hemagglutinin in Radioimmunoassay applications (William, W.V., et. al. (1988). Proc Natl Acad Sci U S A. 85(17):6488-92).

      Flow Cytometry Analysis: A representative lot detected Reovirus Type 3 sigma 1 hemagglutinin in Flow Cytometry applications (Dietrich, M.H., et. al. (2017). J Virol. 91(4). pii: e01621-16).

      Neutralizing Analysis: A representative lot neutralized Reovirus Type 3 in Neutralizing applications (William, W.V., et. al. (1988). Proc Natl Acad Sci USA. 85(17):6488-92).
      Biological Information
      ImmunogenPurified reovirus type 3 virons.
      Clone9BG5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus (Reovirus type 3).
      Specific ActivityClone 9BG5 detects hemaglutinin protein of type 3 reovirus. Specific for the sigma 1 hemagglutinin of Type 3 Reovirus. It neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightN/A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB994-I-25UG 04054839643002

      Documentation