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MABS1223 Anti-SUMO-1 Antibody, clone 4D12

Detect SUMO1 and SUMP1-modified (SUMOylated) proteins using this rat monoclonal Anti-SUMO-1, clone 4D12, Cat. No. MABS1223, validated for use in Immunocytochemistry and Western Blotting.

Anti-SUMO-1 Antibody, clone 4D12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SUMO1, SMT3C, SMT3H3, UBL1, OK/SW-cl.43 UniProt Number: P63165
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MABS1223
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICCRUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1223
      DescriptionAnti-SUMO-1 Antibody, clone 4D12
      Alternate Names
      • mall ubiquitin-related modifier 1
      • GAP-modifying protein 1
      • GMP1
      • Sentrin
      • SMT3 homolog 3
      • Ubiquitin-homology domain protein PIC1
      • Smt3C
      • SUMO-1
      • Ubiquitin-like protein SMT3C
      • Ubiquitin-like protein UBL1
      Background InformationSmall ubiquitin-related modifier 1 (UniProt P63165, P63166, Q5I0H3; also known as GAP-modifying protein 1, GMP1, Sentrin, SMT3 homolog 3, Ubiquitin-homology domain protein PIC1, Smt3C, SUMO-1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1) is encoded by the human SUMO1 (also known as SMT3C, SMT3H3, UBL1,OK/SW-cl.43), mouse & rat Sumo1 (also known as Smt3c, Smt3h3, Ubl1) gene (Gene ID 7341, 22218, 301442, respectively). SUMOylation, protein posttranslation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). As is the case with ubiquitination, SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the deSUMOylation. SUMOylation usually occurs at lysine residues in the consensus ΨKxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares approximately 50% and SUMO4 shares about 87% identity with SUMO2/3. SUMO4 is the only SUMO member whose ctivation and conjugation has not been demonstrated. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator.
      References
      Product Information
      FormatUnpurified
      PresentationRat monoclonal hybridoma culture supernatant with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationDetect SUMO1 and SUMP1-modified (SUMOylated) proteins using this rat monoclonal Anti-SUMO-1, clone 4D12, Cat. No. MABS1223, validated for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected SUMO-1 nuclear puncta enriched in heterochromatin and co-localized with SUMO-2/3 immunoreactivity by indirect fluorescence staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized C-33A human cervical carcinoma cells (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).

      Western Blotting Analysis: A representative lot detected SUMO-1 and SUMO-1-modified (SUMOylated) proteins in human cervical carcinoma C-33A nuclear extract (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).
      Biological Information
      ImmunogenGST-tagged recombinant full-length human SUMO-1.
      Clone4D12
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 4D12 detects SUMO-1 and SUMO-1-modified (SUMOylated) proteins.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. SUMO-1 sequence is 100% conserved among canine, human, murine, and rat species. Predicted to react with a broad-range of mammalian species based on high sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SUMO1
      • SMT3C
      • SMT3H3
      • UBL1
      • OK/SW-cl.43
      Purification MethodUnpurified.
      UniProt Number
      Molecular WeightVariable depending on the size(s) of the SUMOylated protein(s). 11.00 kDa (human/mouse/rat mature SUMO-1; a.a. 2-97) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this hybridoma culture supernatant detected SUMO-1-modified (SUMOylated) proteins in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1223 04054839081323