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IM63 Anti-TIMP-1 (Ab-4) Mouse mAb (102D1)

Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tissue Inhibitor of Metalloproteinase-1

IM63
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~28.5 kDa TIMP-1 protein in endothelial cells, fibroblasts, and breast carcinoma tissue.
      Catalogue NumberIM63
      Brand Family Calbiochem®
      SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
      Application Data
      Detection of human TIMP-1 by immunohistochemistry. Sample: Human breast carcinoma tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: fluorescence.

      Detection of human TIMP-1 by immunoblotting. Sample: Human TIMP-1. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHerbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
      Jung, K., et al. 1997. Int. J. Cancer 74, 220.
      Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Positive controlCytotrophoblastic columns, endothelial or fibroblastic stromal cells of villi, large decidualized cells of decidual membrane in placenta, or breast carcinoma
      PreservativeNone
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (1:100)
      Immunoblotting (1:200-1:400, chemiluminescence)
      Paraffin Sections (1:50, heat pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human TIMP-1
      ImmunogenHuman
      Clone102D1
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      IM63 0

      Documentation

      Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) Certificates of Analysis

      TitleLot Number
      IM63

      References

      Reference overview
      Herbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
      Jung, K., et al. 1997. Int. J. Cancer 74, 220.
      Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-February-2009 JSW
      SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
      ApplicationFrozen Sections (1:100)
      Immunoblotting (1:200-1:400, chemiluminescence)
      Paraffin Sections (1:50, heat pre-treatment required)
      Application Data
      Detection of human TIMP-1 by immunohistochemistry. Sample: Human breast carcinoma tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: fluorescence.

      Detection of human TIMP-1 by immunoblotting. Sample: Human TIMP-1. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3-X63-Ag8.653 mouse myeloma cells. Recognizes the ~28-29 kDa TIMP-1 protein.
      BackgroundThe activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9 and MMP-1 interacting through the catalytic domain of the MMP protein. The inhibition is via reversible non-covalent binding to form a 1:1 complex with the MMP and having a Kd of approximately 10-10
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human TIMP-1
      Clone102D1
      IsotypeIgG₁
      Specieshuman
      Positive controlCytotrophoblastic columns, endothelial or fibroblastic stromal cells of villi, large decidualized cells of decidual membrane in placenta, or breast carcinoma
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHerbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
      Jung, K., et al. 1997. Int. J. Cancer 74, 220.
      Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.