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MABC1765-25UL Anti-TfR1 (CD71) Antibody, clone 3F3-FMA

Anti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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MABC1765-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1765-25UL
      DescriptionAnti-TfR1 (CD71) Antibody, clone 3F3-FMA
      Alternate Names
      • TR
      • TfR
      • TfR1
      • Trfr
      • T9
      • p90
      • CD71
      Background InformationTransferrin receptor protein 1 (UniProt: Q62351; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the Tfrc (also known as Trfr) gene (Gene ID: 7037) in human. TfR1 is a single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-67), a transmembrane domain (aa 68-88), and a long extracellular domain (aa 89-760). It can also be proteolytically cleaved at Arg100 to generate the soluble serum form (sTfR). TfR1 is involved in the uptake of iron via receptor mediated endocytosis into specialized endosomes where acidification leads to release of iron. TfR1 is reported to be essential for erythrocyte development and positive regulation of T and B cell proliferation through iron uptake. It also acts as a lipid sensor that regulates mitochondrial fusion by regulating the activation of the JNK pathway and degradation of mitofusin MFN2. Under conditions of low dietary levels of stearate (C18:0), it promotes the activation of JNK pathway that results in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2 and inhibition of mitochondrial fusion. Clone 3F3-FMA is reported to be an effective ferroptosis-staining agent. It stains plasma membrane and the perinuclear region associated with the Golgi and the endosomal recycling compartment. (Ref.: Senilymaz, D., et al. (2015). Nature. 525(7567); 124-128; Feng, H., et al. (2020). Cell Rep. 30(10); 3411-3423).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesFlow Cytometry Analysis: A representative lot detected TfR1 (CD71) in Flow Cytometry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TfR1 (CD71) in HT-1080 cells.

      Immunohistochemistry Applications: A representative lot detected TfR1 (CD71) in Immunohistochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Immunocytochemistry Analysis: A representative lot detected TfR1 (CD71) in Immunocytochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Western Blotting Analysis: A representative lot detected TfR1 (CD71) in Western Blotting applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified membrane fractions from OCI-LY7 cells.
      EpitopeUnknown
      Clone3F3-FMA
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3F3-FMA is a mouse monoclonal antibody that detects human Transferrin receptor protein 1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tfrc
      • Trfr
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~90 kDa observed; 84.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-1080 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected TfR1 (CD71) in HT-1080 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1765-25UL 04065265944670

      Documentation

      Anti-TfR1 (CD71) Antibody, clone 3F3-FMA SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TfR1 (CD71) Antibody, clone 3F3-FMA Certificates of Analysis

      TitleLot Number
      Anti-TfR1 (CD71), clone 3F3-FMA - 3794324 3794324
      Anti-TfR1 (CD71), clone 3F3-FMA - 3800107 3800107
      Anti-TfR1 (CD71), clone 3F3-FMA - 3802600 3802600
      Anti-TfR1 (CD71), clone 3F3-FMA - 3804073 3804073
      Anti-TfR1 (CD71), clone 3F3-FMA - 3816718 3816718
      Anti-TfR1 (CD71), clone 3F3-FMA - 3819162 3819162
      Anti-TfR1 (CD71), clone 3F3-FMA - 3846469 3846469
      Anti-TfR1 (CD71), clone 3F3-FMA - 3874863 3874863
      Anti-TfR1 (CD71), clone 3F3-FMA - 3884792 3884792
      Anti-TfR1 (CD71), clone 3F3-FMA - 3885548 3885548
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