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OP121 Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16)

Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Erythroblastosis Virus, Anti-HER4, Anti-ErbB4

OP121
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~180-185 kDa c-ErbB4 protein in MDA-MB-453 and T47D cells. Does not cross-react with c-ErbB1, c-ErbB2/c-Neu, or c-ErbB3.
      Catalogue NumberOP121
      Brand Family Calbiochem®
      SynonymsAnti-Erythroblastosis Virus, Anti-HER4, Anti-ErbB4
      References
      ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Carraway, K.L., et al. 1994. J. Biol. Chem. 269, 14303.
      Tzahar, E., et al. 1994. J. Biol. Chem. 269, 25226.
      Plowman, G.D., et al. 1993. Nature 366, 473.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlMDA-MB-453 or T-47D cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesOriginal Clone Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Key Applications Flow Cytometry
      Immunoprecipitation
      Not Western Blot
      Application NotesFlow Cytometry (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Immunoblotting (not recommended)
      Application Commentsc-erbB4 exists as two alternatively-spliced isoforms. Does not cross react with c-erbB1 (EGFR), c-erbB2/HER-2 or c-erbB3/HER-3. This antibody does not interfere with the receptor-heregulin binding and is particularly well suited for immunoprecipitation of unoccupied as well as the occupied c-erbB4 receptor. It reacts with intact cells, stimulates tyrosine kinase activity and efficiently stimulates internalization of c-erbB4 (use antibody at 10 µg/ml). This antibody has also been shown to work for flow cytometry. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of the extracellular domain of human c-erbB4
      ImmunogenHuman
      CloneH4.77.16
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP121 0

      Documentation

      Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-ErbB4 (Ab-1) Mouse mAb (H4.77.16) Certificates of Analysis

      TitleLot Number
      OP121

      References

      Reference overview
      Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Carraway, K.L., et al. 1994. J. Biol. Chem. 269, 14303.
      Tzahar, E., et al. 1994. J. Biol. Chem. 269, 25226.
      Plowman, G.D., et al. 1993. Nature 366, 473.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      SynonymsAnti-Erythroblastosis Virus, Anti-HER4, Anti-ErbB4
      ApplicationFlow Cytometry (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Immunoblotting (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma NSO cells (see application references. Recognizes the ~180-185 kDa erbB4/HER-4 protein.
      Backgroundc-erbB4/HER-4 is the fourth member of the type I transmembrane tyrosine kinase family of growth factor receptors that includes c-erbB1 (EGFR), an orphan receptor, c-erbB2 and c-erbB3, a receptor for the neu differentiation factor (NDF/heregulin). Like c-erbB3, c-erbB4 is also a receptor for NDF/heregulin. Each receptor member is characterized by ectodomains with two cysteine rich regions however, despite their structural homology, they differ in ligand specificities. c-erbB1/Her-1 binds EGF whereas c-erbB3 and c-erbB4 are the respective low and high affinity receptors for many isoforms of NDF/heregulin. Currently the ligand specificity for c-erbB2 has not been well characterized and thus remains the 'orphan receptor'. All four receptor members are expressed in breast cancer presumably a result of gene amplification. Overexpression of perhaps all of the receptors can also occur, probably as a result of increased mRNA transcription.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of the extracellular domain of human c-erbB4
      CloneH4.77.16
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlMDA-MB-453 or T-47D cells
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      Commentsc-erbB4 exists as two alternatively-spliced isoforms. Does not cross react with c-erbB1 (EGFR), c-erbB2/HER-2 or c-erbB3/HER-3. This antibody does not interfere with the receptor-heregulin binding and is particularly well suited for immunoprecipitation of unoccupied as well as the occupied c-erbB4 receptor. It reacts with intact cells, stimulates tyrosine kinase activity and efficiently stimulates internalization of c-erbB4 (use antibody at 10 µg/ml). This antibody has also been shown to work for flow cytometry. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Carraway, K.L., et al. 1994. J. Biol. Chem. 269, 14303.
      Tzahar, E., et al. 1994. J. Biol. Chem. 269, 25226.
      Plowman, G.D., et al. 1993. Nature 366, 473.
      Application referencesOriginal Clone Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.