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OP31 Anti-c-Myc (Ab-3) Mouse mAb (33)

Anti-c-Myc (Ab-3) Mouse mAb (33) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP31
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Av, H, MMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~60-67 kDa c-Myc protein HL60 cells.
      Catalogue NumberOP31
      Brand Family Calbiochem®
      References
      ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
      Nau, M.N., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Dalla-Favera, R., et al. 1983. Science 219, 963.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlHT1080 cells
      Positive controlHL-60 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Wafa, O., et al. 2002. Mol. Cell 9, 1031. Paraffin Sections Reichelt, J. and Magin, T.M. 2002. J. Cell Sci. 115, 2639.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Not Paraffin Sections
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (see comments and application references)
      Application CommentsThe c-myc protein is extremely labile. c-myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. Recognizes full-length c-myc as well as c-myc cleavage products. The sequence of c-myc predicts a ~45 kDa protein, but c-myc migrates under reducing conditions as a ~64-67 kDa band. For staining paraffin sections, heat pretreatment in 10 mM citrate buffer, pH 6.0 is required (see application references). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human c-Myc
      Clone33
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Avian
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP31 0

      Documentation

      Anti-c-Myc (Ab-3) Mouse mAb (33) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-Myc (Ab-3) Mouse mAb (33) Certificates of Analysis

      TitleLot Number
      OP31

      References

      Reference overview
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
      Nau, M.N., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Dalla-Favera, R., et al. 1983. Science 219, 963.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-August-2008 JSW
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (see comments and application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells. Recognizes the ~60-67 kDa (apparent MW) c-Myc protein.
      BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. In both mouse plasmacytomas and human Burkitt's lymphoma, coding sequences from the c-myc gene are translocated from their normal chromosomal position into a transcriptionally active immunoglobulin locus on another chromosome. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Furthermore, elevated levels of myc mRNA and protein have been demonstrated in human tumors even in the absence of gene amplification. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
      HostMouse
      Immunogenrecombinant, human c-Myc
      Clone33
      IsotypeIgG₁
      Speciesavian, human, mouse
      Positive controlHL-60 cells
      Negative controlHT1080 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe c-myc protein is extremely labile. c-myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. Recognizes full-length c-myc as well as c-myc cleavage products. The sequence of c-myc predicts a ~45 kDa protein, but c-myc migrates under reducing conditions as a ~64-67 kDa band. For staining paraffin sections, heat pretreatment in 10 mM citrate buffer, pH 6.0 is required (see application references). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
      Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
      Nau, M.N., et al. 1985. Nature 318, 69.
      Persson, H., et al. 1984. Science 225, 687.
      Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
      Dalla-Favera, R., et al. 1983. Science 219, 963.
      Application referencesImmunoblotting Wafa, O., et al. 2002. Mol. Cell 9, 1031. Paraffin Sections Reichelt, J. and Magin, T.M. 2002. J. Cell Sci. 115, 2639.