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04-1462 Anti-hnRNP F Antibody, clone 3H4

Use Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F.

Anti-hnRNP F Antibody, clone 3H4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: HNRPF, hnRNP F UniProt Number: P52597
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04-1462
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1462
      DescriptionAnti-hnRNP F Antibody, clone 3H4
      Alternate Names
      • heterogeneous nuclear ribonucleoprotein F
      • nucleolin-like protein
      • HnRNP F protein
      • Nucleolin-like protein mcs94-1
      • mcs94-1
      Background InformationThe hnRNP F protein is member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
      The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. There are more than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) associated with pre-mRNAs in the nucleus of mammalian cells. These different hnRNPs display a range of affinities for a variety of ribonucleotidehomopolymers and ssDNA sequences. In constrast, hnRNP F protein as well as hnRNP H (a highly related protein) appear to bind only to poly(rG). These unique RNA-binding properties, as well as the amino acid sequence and other data showing a distinct intranuclear localization of hnRNP F and hnRNP H suggest they are unique from other hnRNP and play an important role in the processing of RNAs containing guanosine-rich sequences.
      References
      Product Information
      FormatPurified
      Control
      • K562 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis:
      A representative lot was used by an independent laboratory for IP. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)

      Immunofluorescence Analysis:
      A representative lot was used by an independent laboratory in IF. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)
      Biological Information
      ImmunogenRecombinant protein corresponding to human hnRNP F.
      EpitopeUnknown
      Cloneclone 3H4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes hnRNP F.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq].
      Gene Symbol
      • HNRPF
      • hnRNP F
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Probably binds G-rich sequences in pre-mRNAs.

      SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRNPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with TBP and TXNL4/DIM1.

      SUBCELLULAR LOCATION: Nucleus › nucleoplasm.

      TISSUE SPECIFICITY: Expressed ubiquitously.

      DOMAIN: The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

      Sumoylated.

      SEQUENCE SIMILARITIES: Contains 3 RRM (RNA recognition motif) domains.
      Molecular WeightCalculated: 46 kDa. Observed: ~53 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected hnRNP F on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      04-1462 04053252478550