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PC435 Anti-p19ARF (54-75) (Ab-1) Rabbit pAb

This Anti-p19ARF (54-75) (Ab-1) Rabbit pAb is validated for use in Immunoblotting for the detection of p19ARF (54-75) (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC435
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      MRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~19 kDa p19ARF protein in CTLL2 cells and mouse embryo fibroblasts.
      Catalogue NumberPC435
      Brand Family Calbiochem®
      Application Data
      Detection of mouse p19ARF by immunoblotting. Sample: Whole cell lysate from wild-type mouse embryonic fibroblasts (lanes 1 and 2) and whole cell lysate from mouse embtyonic fibroblasts bearing a p19ARF gene knockout as a negative control (lane 3). Primary antibody: Anti-p19ARF (54-75) (Ab-1) Rabbit pAb (Cat. No. PC435) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesDe Stanchina, E., et al. 1998. Genes Dev. 12, 2434.
      Larson, C.J., 1998. Bull. Cancer. 85, 304.
      Radfar, A., et al. 1998. Proc. Natl. Acad Sci USA. 95, 13194.
      Zindy, F., et al. 1998. Genes Dev. 12, 2424.
      Product Information
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Positive controlMouse embryo fibroblasts or CTLL2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.5-1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 54-75 of mouse p19ARF, conjugated to KLH
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PC435 0

      Documentation

      Anti-p19ARF (54-75) (Ab-1) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p19ARF (54-75) (Ab-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC435

      References

      Reference overview
      De Stanchina, E., et al. 1998. Genes Dev. 12, 2434.
      Larson, C.J., 1998. Bull. Cancer. 85, 304.
      Radfar, A., et al. 1998. Proc. Natl. Acad Sci USA. 95, 13194.
      Zindy, F., et al. 1998. Genes Dev. 12, 2424.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-October-2007 RFH
      ApplicationImmunoblotting (0.5-1 µg/ml)
      Application Data
      Detection of mouse p19ARF by immunoblotting. Sample: Whole cell lysate from wild-type mouse embryonic fibroblasts (lanes 1 and 2) and whole cell lysate from mouse embtyonic fibroblasts bearing a p19ARF gene knockout as a negative control (lane 3). Primary antibody: Anti-p19ARF (54-75) (Ab-1) Rabbit pAb (Cat. No. PC435) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~19 kDa p19ARF protein.
      BackgroundIn humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependant pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression. p19ARF recognizes in vitro translated murine p19ARF and a ~19 kDa band in murine CTLL2 cells.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide corresponding to amino acids 54-75 of mouse p19ARF, conjugated to KLH
      IsotypeIgG
      Speciesmouse
      Positive controlMouse embryo fibroblasts or CTLL2 cells
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDe Stanchina, E., et al. 1998. Genes Dev. 12, 2434.
      Larson, C.J., 1998. Bull. Cancer. 85, 304.
      Radfar, A., et al. 1998. Proc. Natl. Acad Sci USA. 95, 13194.
      Zindy, F., et al. 1998. Genes Dev. 12, 2424.