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OP09L Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801)

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OP09L
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~53 kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
      Catalogue NumberOP09L
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlA431 cells or breast carcinoma tissue
      PreservativeNone
      Applications
      Application ReferencesOriginal Clone Banks, L., et al. 1986. Eur. J. Biochem. 159, 529. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071.
      Key Applications Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Gel Shift (see comments)
      Immunoblotting (2.5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
      Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with 35S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using PC35 and chemiluminescent detection. For a gel shift assay, resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant p53 fusion protein
      ImmunogenHuman
      Epitopewithin amino acids 46-55 of human p53
      ClonePAb1801
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP09L 0

      Documentation

      Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) Certificates of Analysis

      TitleLot Number
      OP09L

      References

      Reference overview
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationFrozen Sections (5 µg/ml)
      Gel Shift (see comments)
      Immunoblotting (2.5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
      BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant p53 fusion protein
      Epitopewithin amino acids 46-55 of human p53
      ClonePAb1801
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlA431 cells or breast carcinoma tissue
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsWild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with 35S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using PC35 and chemiluminescent detection. For a gel shift assay, resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
      Application referencesOriginal Clone Banks, L., et al. 1986. Eur. J. Biochem. 159, 529. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies