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OP43L Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Gel Shift, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP43L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Fe, HMMonoclonal Antibody

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OP43L-100UG
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      Description
      OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
      Catalogue NumberOP43L
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
      Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlSK-OV-3 cells or normal skin tissue
      Positive controlA431 cells or breast carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation, Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.
      Key Applications Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen sections (1 µg/ml; see application references)
      Gel Shift (see comments)
      Immunoblotting (0.1 µg/ml; see application references)
      Immunocytochemistry (1-2.5 µg/ml; see application references)
      Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)
      Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
      Application CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, resuspend in 100 µl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenwild type, recombinant, human p53
      ImmunogenHuman
      EpitopeWithin amino acids 21-25 of human p53
      CloneDO-1
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Feline
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 μg/ml. Reconstituted product will be more stable if 0.1% sodium azide is added (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two hours. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP43L-100UG 04055977227536

      Documentation

      Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Certificates of Analysis

      TitleLot Number
      OP43L

      References

      Reference overview
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
      Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

      Citations

      Title
    • Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.
    • Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.
    • Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      ApplicationFrozen sections (1 µg/ml; see application references)
      Gel Shift (see comments)
      Immunoblotting (0.1 µg/ml; see application references)
      Immunocytochemistry (1-2.5 µg/ml; see application references)
      Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)
      Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
      BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.
      HostMouse
      Immunogen speciesHuman
      Immunogenwild type, recombinant, human p53
      EpitopeWithin amino acids 21-25 of human p53
      CloneDO-1
      IsotypeIgG2a
      Speciesfeline, human, not mouse, not rat
      Positive controlA431 cells or breast carcinoma tissue
      Negative controlSK-OV-3 cells or normal skin tissue
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, resuspend in 100 µl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 μg/ml. Reconstituted product will be more stable if 0.1% sodium azide is added (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two hours. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
      Toxicity Standard Handling
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
      Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
      Citation
    • Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.
    • Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.
    • Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
      		•
      Application referencesImmunoprecipitation, Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies