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OP43A Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate

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OP43A
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Fe, HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
      Catalogue NumberOP43A
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
      Product Information
      FormLiquid slurry
      FormulationAgarose suspension in PBS.
      Negative controlSK-OV-3 cells
      Positive controlA431 cells
      Preservative≤ 0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071. Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
      Key Applications Affinity Chromatography
      Immunoprecipitation
      Application NotesAffinity Chromatography (see comments)
      Immunoprecipitation (15 µl beads/sample; see application references)
      Application CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenwild type, recombinant, human p53
      ImmunogenHuman
      EpitopeWithin amino acids 21-25 of human p53
      CloneDO-1
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Feline
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP43A 0

      Documentation

      Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Agarose Conjugate Certificates of Analysis

      TitleLot Number
      OP43A

      References

      Reference overview
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      ApplicationAffinity Chromatography (see comments)
      Immunoprecipitation (15 µl beads/sample; see application references)
      DescriptionImmunoaffinity purified mouse monoclonal antibody, generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with X63AG8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
      BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.
      HostMouse
      Immunogen speciesHuman
      Immunogenwild type, recombinant, human p53
      EpitopeWithin amino acids 21-25 of human p53
      CloneDO-1
      IsotypeIgG2a
      Speciesfeline, human, not mouse, not rat
      Positive controlA431 cells
      Negative controlSK-OV-3 cells
      FormLiquid slurry
      FormulationAgarose suspension in PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤ 0.1% sodium azide
      CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Agarose conjugate has also been reported to work with affinity chromotography. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
      Application referencesImmunoprecipitation Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071. Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies