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04-294 Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5

Detect phosho-erbB2/Her2 (Tyr1112) using this Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5 validated for use in ELISA & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: ERBB2, HER2, HER-2, NGL, herstatin, erbB-2, C-erbB-2, NEU, TKR1, p185erbB2, HER-2/neu UniProt Number: P04626
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04-294
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, RELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-294
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5
      References
      Product Information
      FormatPurified
      Control
      • Includes EGF treated SKOV-3 lysate as a positive control
      PresentationLyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
      Quality LevelMQ100
      Applications
      ApplicationDetect phosho-erbB2/Her2 (Tyr1112) using this Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5 validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr1112 in human erbB2/Her2
      Clone19G5
      HostMouse
      SpecificityRecognizes phosphorylated domain of erbB2/Her2 on Tyrosine 1112
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
      Gene Symbol
      • ERBB2
      • HER2
      • HER-2
      • NGL
      • herstatin
      • erbB-2
      • C-erbB-2
      • NEU
      • TKR1
      • p185erbB2
      • HER-2/neu
      Modifications
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
      SIZE: 1255 amino acids; 137910 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
      SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      04-294 04053252367526

      Documentation

      Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5 Certificates of Analysis

      TitleLot Number
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 (Mouse monoclonal IgG1) - 2454690 2454690
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 (Mouse monoclonal IgG1) 2967801
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 (Mouse monoclonal IgG1) 2943145
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 - 2383445 2383445
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 - 0216S0102 0216S0102
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 - 2914554 2914554
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2586411 2586411
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2617718 2617718
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2638898 2638898
      Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2817742 2817742
      Show more

      References

      Reference overviewPub Med ID
      Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2.
      Klapper, L N, et al.
      Cancer Res., 60: 3384-8 (2000)  2000

      Show Abstract       		10910043 10910043

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies