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MABS155 Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Hormones & Receptors Gene Symbol: ADRBK1, BARK, BARK1, GRK2 UniProt Number: P25098
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MABS155
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, PIAMUnconjugatedMonoclonal Antibody
      Description
      Catalogue NumberMABS155
      DescriptionAnti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1
      Alternate Names
      • Beta-adrenergic receptor kinase 1
      • Beta-ARK-1
      • G-protein coupled receptor kinase 2
      Background InformationG-protein coupled receptor kinase 2 (Grk2), also known as BARK or BARK1, is a member of the large family of GRK kinases that phosphorylate G-protein coupled receptors (GPCR), resulting in the recruitment of arrestins to the cytoplasmic domains of GPCR’s and subsequent internalization of these receptors by endocytosis. GRK2 is ubiquitously expressed, and modulates diverse cellular processes through association with a number of non-receptor substrates. GRK2 regulates actin dynamics and cell motililty through interaction with radixin, tubilin, and ezrin. In addition, Grk2 may play a role in the signaling of TGFβ by blocking the nuclear translocation of Smad2 and Smad3 proteins. It has also been shown that Grk2 may play a role in the hedgehog signaling pathway during embryonic development, by modulating the signaling of the atypical G-protein coupled receptor Smo. Grk2 is inactivated by phosphorylation of ser670 by ERK. It is also phosphorylated by PKC and Src, depending on the cellular context.
      References
      Product Information
      FormatUnconjugated
      Control
      • Untreated and Calyculin A/Okadaic Acid treated A431 cells
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot blocked phospho-GRK2 in 10 µg of Calyculin A/Okadaic Acid treated and untreated Jurkat cells.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-GRK2 in HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human GRK2 phosphorylated at Ser670.
      EpitopePhosphorylated Ser670
      Clone15G11.1
      HostMouse
      SpecificityThis antibody recognizes GRK2 phosphorylated at Ser670.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ADRBK1
      • BARK
      • BARK1
      • GRK2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and Calyculin A/Okadaic Acid treated A431 cells.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected GRK2 in 10 µg of untreated and Calyculin A/Okadaic Acid treated A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS155 04053252440854

      Documentation

      Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 Certificates of Analysis

      TitleLot Number
      Anti-phospho-GRK2 (Ser670), -2576404 2576404
      Anti-phospho-GRK2 (Ser670), -2640559 2640559
      Anti-phospho-GRK2 (Ser670), -2686168 2686168
      Anti-phospho-GRK2 (Ser670), -2718264 2718264
      Anti-phospho-GRK2 (Ser670), -2792488 2792488
      Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 2298450 2298450
      Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 3423237 3423237
      Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 4162702 4162702
      Anti-phospho-GRK2 (Ser670), clone 15G11.1 - Q2032354 Q2032354
      Anti-phospho-GRK2 (Ser670), clone 15G11.1 Monoclonal Antibody 3050683

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies